Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-27625 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CIZ1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, HeLa, HepG2, HCT116.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CIZ1 Polyclonal Antibody detects CIZ1 protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract.7.5 % SDS-PAGE. CIZ1 Polyclonal Antibody (Product # PA5-27625) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CIZ1 in paraformaldehyde-fixed HeLa cells using a CIZ1 polyclonal antibody (Product # PA5-27625) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CIZ1 (Product # PA5-27625) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CIZ1 antibody immunoprecipitates CIZ1 protein in IP experiments. IP Sample: 1,000 µg HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of CIZ1 protein by 2.5 µg of CIZ1 antibody (Product # PA5-27625) 7.5% SDS-PAGE The immunoprecipitated CIZ1 protein was detected by CIZ1 antibody (Product # PA5-27625) diluted at 1:1,000.