Antibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-966 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-966, RRID:AB_628162
- Product name
- Anti-PML
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Functional reorganization of promyelocytic leukemia nuclear bodies during BK virus infection.
SUMO E3 ligase activity of TRIM proteins.
Functional interaction of nuclear domain 10 and its components with cytomegalovirus after infections: cross-species host cells versus native cells.
Cyclin-dependent kinase-like function is shared by the beta- and gamma- subset of the conserved herpesvirus protein kinases.
Quantitative proteomic analysis of A549 cells infected with human respiratory syncytial virus.
Cellular and viral control over the initial events of human cytomegalovirus experimental latency in CD34+ cells.
Co-localization of the amyloid precursor protein and Notch intracellular domains in nuclear transcription factories.
Novel nuclear nesprin-2 variants tether active extracellular signal-regulated MAPK1 and MAPK2 at promyelocytic leukemia protein nuclear bodies and act to regulate smooth muscle cell proliferation.
SENP3-mediated de-conjugation of SUMO2/3 from promyelocytic leukemia is correlated with accelerated cell proliferation under mild oxidative stress.
Novel snail1 target proteins in human colon cancer identified by proteomic analysis.
The G-quadruplex ligand telomestatin impairs binding of topoisomerase IIIalpha to G-quadruplex-forming oligonucleotides and uncaps telomeres in ALT cells.
Isolation of cell nuclei using inert macromolecules to mimic the crowded cytoplasm.
BRAF(V600E) efficient transformation and induction of microsatellite instability versus KRAS(G12V) induction of senescence markers in human colon cancer cells.
The two functions of herpes simplex virus 1 ICP0, inhibition of silencing by the CoREST/REST/HDAC complex and degradation of PML, are executed in tandem.
Mitochondrial dysfunction contributes to oncogene-induced senescence.
Human SRY inhibits beta-catenin-mediated transcription.
Histone deacetylase 7 promotes PML sumoylation and is essential for PML nuclear body formation.
Small ubiquitin-related modifier (SUMO) binding determines substrate recognition and paralog-selective SUMO modification.
SUMO conjugation to the matrix attachment region-binding protein, special AT-rich sequence-binding protein-1 (SATB1), targets SATB1 to promyelocytic nuclear bodies where it undergoes caspase cleavage.
Signal-dependent regulation of transcription by histone deacetylase 7 involves recruitment to promyelocytic leukemia protein nuclear bodies.
PDLIM2-mediated termination of transcription factor NF-κB activation by intranuclear sequestration and degradation of the p65 subunit
Jiang M, Entezami P, Gamez M, Stamminger T, Imperiale MJ
mBio 2011;2(1):e00281-10
mBio 2011;2(1):e00281-10
SUMO E3 ligase activity of TRIM proteins.
Chu Y, Yang X
Oncogene 2011 Mar 3;30(9):1108-16
Oncogene 2011 Mar 3;30(9):1108-16
Functional interaction of nuclear domain 10 and its components with cytomegalovirus after infections: cross-species host cells versus native cells.
Cosme RC, Martínez FP, Tang Q
PloS one 2011 Apr 28;6(4):e19187
PloS one 2011 Apr 28;6(4):e19187
Cyclin-dependent kinase-like function is shared by the beta- and gamma- subset of the conserved herpesvirus protein kinases.
Kuny CV, Chinchilla K, Culbertson MR, Kalejta RF
PLoS pathogens 2010 Sep 9;6(9)
PLoS pathogens 2010 Sep 9;6(9)
Quantitative proteomic analysis of A549 cells infected with human respiratory syncytial virus.
Munday DC, Emmott E, Surtees R, Lardeau CH, Wu W, Duprex WP, Dove BK, Barr JN, Hiscox JA
Molecular & cellular proteomics : MCP 2010 Nov;9(11):2438-59
Molecular & cellular proteomics : MCP 2010 Nov;9(11):2438-59
Cellular and viral control over the initial events of human cytomegalovirus experimental latency in CD34+ cells.
Saffert RT, Penkert RR, Kalejta RF
Journal of virology 2010 Jun;84(11):5594-604
Journal of virology 2010 Jun;84(11):5594-604
Co-localization of the amyloid precursor protein and Notch intracellular domains in nuclear transcription factories.
Konietzko U, Goodger ZV, Meyer M, Kohli BM, Bosset J, Lahiri DK, Nitsch RM
Neurobiology of aging 2010 Jan;31(1):58-73
Neurobiology of aging 2010 Jan;31(1):58-73
Novel nuclear nesprin-2 variants tether active extracellular signal-regulated MAPK1 and MAPK2 at promyelocytic leukemia protein nuclear bodies and act to regulate smooth muscle cell proliferation.
Warren DT, Tajsic T, Mellad JA, Searles R, Zhang Q, Shanahan CM
The Journal of biological chemistry 2010 Jan 8;285(2):1311-20
The Journal of biological chemistry 2010 Jan 8;285(2):1311-20
SENP3-mediated de-conjugation of SUMO2/3 from promyelocytic leukemia is correlated with accelerated cell proliferation under mild oxidative stress.
Han Y, Huang C, Sun X, Xiang B, Wang M, Yeh ET, Chen Y, Li H, Shi G, Cang H, Sun Y, Wang J, Wang W, Gao F, Yi J
The Journal of biological chemistry 2010 Apr 23;285(17):12906-15
The Journal of biological chemistry 2010 Apr 23;285(17):12906-15
Novel snail1 target proteins in human colon cancer identified by proteomic analysis.
Larriba MJ, Casado-Vela J, Pendás-Franco N, Peña R, García de Herreros A, Berciano MT, Lafarga M, Casal JI, Muñoz A
PloS one 2010 Apr 20;5(4):e10221
PloS one 2010 Apr 20;5(4):e10221
The G-quadruplex ligand telomestatin impairs binding of topoisomerase IIIalpha to G-quadruplex-forming oligonucleotides and uncaps telomeres in ALT cells.
Temime-Smaali N, Guittat L, Sidibe A, Shin-ya K, Trentesaux C, Riou JF
PloS one 2009 Sep 9;4(9):e6919
PloS one 2009 Sep 9;4(9):e6919
Isolation of cell nuclei using inert macromolecules to mimic the crowded cytoplasm.
Hancock R, Hadj-Sahraoui Y
PloS one 2009 Oct 23;4(10):e7560
PloS one 2009 Oct 23;4(10):e7560
BRAF(V600E) efficient transformation and induction of microsatellite instability versus KRAS(G12V) induction of senescence markers in human colon cancer cells.
Oikonomou E, Makrodouli E, Evagelidou M, Joyce T, Probert L, Pintzas A
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1116-31
Neoplasia (New York, N.Y.) 2009 Nov;11(11):1116-31
The two functions of herpes simplex virus 1 ICP0, inhibition of silencing by the CoREST/REST/HDAC complex and degradation of PML, are executed in tandem.
Gu H, Roizman B
Journal of virology 2009 Jan;83(1):181-7
Journal of virology 2009 Jan;83(1):181-7
Mitochondrial dysfunction contributes to oncogene-induced senescence.
Moiseeva O, Bourdeau V, Roux A, Deschênes-Simard X, Ferbeyre G
Molecular and cellular biology 2009 Aug;29(16):4495-507
Molecular and cellular biology 2009 Aug;29(16):4495-507
Human SRY inhibits beta-catenin-mediated transcription.
Bernard P, Sim H, Knower K, Vilain E, Harley V
The international journal of biochemistry & cell biology 2008;40(12):2889-900
The international journal of biochemistry & cell biology 2008;40(12):2889-900
Histone deacetylase 7 promotes PML sumoylation and is essential for PML nuclear body formation.
Gao C, Ho CC, Reineke E, Lam M, Cheng X, Stanya KJ, Liu Y, Chakraborty S, Shih HM, Kao HY
Molecular and cellular biology 2008 Sep;28(18):5658-67
Molecular and cellular biology 2008 Sep;28(18):5658-67
Small ubiquitin-related modifier (SUMO) binding determines substrate recognition and paralog-selective SUMO modification.
Zhu J, Zhu S, Guzzo CM, Ellis NA, Sung KS, Choi CY, Matunis MJ
The Journal of biological chemistry 2008 Oct 24;283(43):29405-15
The Journal of biological chemistry 2008 Oct 24;283(43):29405-15
SUMO conjugation to the matrix attachment region-binding protein, special AT-rich sequence-binding protein-1 (SATB1), targets SATB1 to promyelocytic nuclear bodies where it undergoes caspase cleavage.
Tan JA, Sun Y, Song J, Chen Y, Krontiris TG, Durrin LK
The Journal of biological chemistry 2008 Jun 27;283(26):18124-34
The Journal of biological chemistry 2008 Jun 27;283(26):18124-34
Signal-dependent regulation of transcription by histone deacetylase 7 involves recruitment to promyelocytic leukemia protein nuclear bodies.
Gao C, Cheng X, Lam M, Liu Y, Liu Q, Chang KS, Kao HY
Molecular biology of the cell 2008 Jul;19(7):3020-7
Molecular biology of the cell 2008 Jul;19(7):3020-7
PDLIM2-mediated termination of transcription factor NF-κB activation by intranuclear sequestration and degradation of the p65 subunit
Takashi Tanaka, Michael J Grusby, Tsuneyasu Kaisho
Nature Immunology 2007 Apr;8(6):584-591
Nature Immunology 2007 Apr;8(6):584-591
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- No bands detected.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 48
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human urinary bladder shows strong nuclear positivity in urothelial cells.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.