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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [5]
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- Product number
- MA5-24604 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WWTR1 Monoclonal Antibody (CL0371)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MNPKPSSWRK KILPESFFKE PDSGSHSRQS STDSSGGHPG PRLAGGAQHV RSHSSPASLQ LGTGAGAAGS PAQQHAHLRQ QSYDVTDELP LPPGWEMTFT ATGQRYFLNH IEKITTWQDP RKAMNQPLNH MNLHPAVSST
- Antibody clone number
- CL0371
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WWTR1 in Lane1: Marker (kDa), Lane 2: Human cell line U-251 MG. Samples were probed using a WWTR1 Monoclonal Antibody (Product # MA5-24604).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WWTR1 in extracts from EFO-21 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using WWTR1 Monoclonal Antibody (CL0371) (Product # MA5-24604). Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence staining of WWTR1 in SKMEL cell line using a WWTR1 Monoclonal Antibody (Product # MA5-24604), showing spotty nuclear staining in green. Microtubule probes are visualized in red (where available).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of WWTR1 in human placenta and lymph node tissues using WWTR1 Monoclonal Antibody (CL0371) (Product # MA5-24604). Corresponding WWTR1 RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of WWTR1 in human breast cancer using WWTR1 Monoclonal Antibody (CL0371) (Product # MA5-24604) shows moderate to strong nuclear positivity in tumor cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of WWTR1 in human placenta using WWTR1 Monoclonal Antibody (CL0371) (Product # MA5-24604) shows strong nuclear and some cytoplasmic positivity in endothelial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of WWTR1 in human kidney using WWTR1 Monoclonal Antibody (CL0371) (Product # MA5-24604) shows strong nuclear and moderate cytoplasmic positivity in cells in tubules and in glomeruli.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of WWTR1 in human lymph node using WWTR1 Monoclonal Antibody (CL0371) (Product # MA5-24604) shows no nuclear positivity in lymphoid cells as expected.
