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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [8]
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- Product number
- PA5-55686 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BCLAF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QKSPEIHRRI DISPSTLRKH TRLAGEERVF KEENQKGDKK LRCDSADLRH DIDRRRKERS KERGDSKGSR ESSGSRKQEK TPKDYKEYKS YKDDSKHKRE QDHSRSSSSS ASPSSPSSR
- Concentration
- 0.08 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BCLAF1 in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using a BCLAF1 Polyclonal Antibody (Product # PA5-55686). Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of BCLAF1 in human cell line A-431 shows positivity in nucleus but excluded from the nucleoli. Samples were probed using a BCLAF1 Polyclonal Antibody (Product # PA5-55686).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of BCLAF1 in human cell line A-431 using a BCLAF1 Polyclonal Antibody (Product # PA5-55686) shows localization to nuclear speckles.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of BCLAF1 in human colon using BCLAF1 Polyclonal Antibody (Product # PA5-55686).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of BCLAF1 in human kidney using BCLAF1 Polyclonal Antibody (Product # PA5-55686).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of BCLAF1 in human bone marrow using BCLAF1 Polyclonal Antibody (Product # PA5-55686).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of BCLAF1 in human bone marrow, cerebral cortex, colon and kidney using BCLAF1 Polyclonal Antibody (Product # PA5-55686) (A) shows similar protein distribution across tissues to an independent BCLAF1 Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of BCLAF1 in human lymphoid tissues using BCLAF1 Polyclonal Antibody (Product # PA5-55686) shows strong nuclear positivity in lymphocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of BCLAF1 in human testis using BCLAF1 Polyclonal Antibody (Product # PA5-55686) shows strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of BCLAF1 in human cerebral cortex using BCLAF1 Polyclonal Antibody (Product # PA5-55686) shows strong nuclear positivity in neurons and glial cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of BCLAF1 in human gastrointestinal using BCLAF1 Polyclonal Antibody (Product # PA5-55686) shows strong nuclear positivity in glandular cells.
