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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00009260-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009260-M01, RRID:AB_546927
- Product name
- Mouse Monoclonal PDLIM7 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PDLIM7 - PDZ and LIM domain 7 (enigma)
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Postsynaptic PDLIM5/Enigma Homolog binds SPAR and causes dendritic spine shrinkage.
Herrick S, Evers DM, Lee JY, Udagawa N, Pak DT
Molecular and cellular neurosciences 2010 Feb;43(2):188-200
Molecular and cellular neurosciences 2010 Feb;43(2):188-200
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of PDLIM7 over-expressed 293 cell line, cotransfected with PDLIM7 Validated Chimera RNAi ( Cat # H00009260-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PDLIM7 monoclonal antibody (M01), clone 2D6 (Cat # H00009260-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of PDLIM7 expression in transfected 293T cell line by PDLIM7 monoclonal antibody (M01), clone 2D6.Lane 1: PDLIM7 transfected lysate(49.8 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PDLIM7 Antibody (2D6) [H00009260-M01] - PDLIM7 monoclonal antibody (M01), clone 2D6 Analysis of PDLIM7 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: PDLIM7 Antibody (2D6) [H00009260-M01] - Detection limit for recombinant GST tagged PDLIM7 is approximately 0.3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of monoclonal antibody to PDLIM7 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PDLIM7 Antibody (2D6) [H00009260-M01] - Analysis of PDLIM7 transfected lysate using anti-PDLIM7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PDLIM7 MaxPab rabbit polyclonal antibody.
