Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
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Validation data
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- Product number
- AF3598 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Rex-1/ZFP42 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human Rex-1/ZFP42 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human YYI is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
NP_777560
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Hyaluronan-CD44 interaction activates stem cell marker Nanog, Stat-3-mediated MDR1 gene expression, and ankyrin-regulated multidrug efflux in breast and ovarian tumor cells.
Bourguignon LY, Peyrollier K, Xia W, Gilad E
The Journal of biological chemistry 2008 Jun 20;283(25):17635-51
The Journal of biological chemistry 2008 Jun 20;283(25):17635-51
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Rex-1/ZFP42 in NTera-2 Human Cell Line. Rex-1/ZFP42 was detected in immersion fixed NTera-2 human testicular embryonic carcinoma cell line using Sheep Anti-Human Rex-1/ZFP42 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3598) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Rex-1/ZFP42 in BG01V Human Stem Cells. Rex-1/ZFP42 was detected in immersion fixed BG01V human embryonic stem cells using 10 µg/mL Sheep Anti-Human Rex-1/ZFP24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3598) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.