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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-90403 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: VIDDESDYFA SDSNQWLSKL ERETLQKREE ELRELRHASR LSKKVTIDFA GRKILEEENS LAEYHSRLDE TIQAIANGTL NQPLTKLDRS SEEPLGVLVN PNMYQSPPQW VDHTGAASQK KAFRSSGFGL EFNSFQHQLR IQDQEFQEGF DGGWCLSVHQ PWASLLVRGI KRVEGRSWYT PHRGRLWIAA TAKKPSPQEV SELQATYRLL RGKDVEFPND YPSGCLLGCV DLIDCLSQKQ FKEQFPDISQ ESDSPFVFIC KNPQEMVVKF PIKGNPKIWK LDSKIHQGAK KGLMKQNKAV; Positive Samples: A-549, HeLa, LO2, Mouse liver, Mouse testis, Mouse brain, Rat testis, Rat brain; Cellular Location: Cytoplasm, Nucleus, centrosome, cytoskeleton, cytosol, microtubule organizing center
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.94 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ASC1 in extracts of various cell lines using ASC1 Polyclonal Antibody (Product # PA5-90403) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ASC1 in paraffin-embedded rat heart using ASC1 Polyclonal Antibody (Product # PA5-90403) at a dilution of 1:200.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ASC1 in paraffin-embedded human colon carcinoma using ASC1 Polyclonal Antibody (Product # PA5-90403) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ASC1 in paraffin-embedded human esophageal using ASC1 Polyclonal Antibody (Product # PA5-90403) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of ASC1 was performed in 200 µg extracts of A-549 cells using ASC1 Polyclonal Antibody (Product # PA5-90403). Western blot was performed from the immunoprecipitate using ASC1 Polyclonal Antibody.
