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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-263-NA - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-1 RII Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-1 RII in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) IL-1 alpha , rhIL-1 RI, recombinant mouse (rm) IL-1 alpha , rhIL-1 beta , rmIL-1 beta , recombinant rat IL-1 beta , rhIL-1ra, rmIL-1ra, and recombinant rhesus monkey IL-1ra is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Ectopic expression of interleukin-1 receptor type II enhances cell migration through activation of the pre-interleukin 1alpha pathway.
Chorionic gonadotropin down-regulates the expression of the decoy inhibitory interleukin 1 receptor type II in human endometrial epithelial cells.
Synergism of TNF and IL-1 in the induction of matrix metalloproteinase-3 in trabecular meshwork.
Chang SY, Su PF, Lee TC
Cytokine 2009 Jan;45(1):32-8
Cytokine 2009 Jan;45(1):32-8
Chorionic gonadotropin down-regulates the expression of the decoy inhibitory interleukin 1 receptor type II in human endometrial epithelial cells.
Herrmann-Lavoie C, Rao CV, Akoum A
Endocrinology 2007 Nov;148(11):5377-84
Endocrinology 2007 Nov;148(11):5377-84
Synergism of TNF and IL-1 in the induction of matrix metalloproteinase-3 in trabecular meshwork.
Kelley MJ, Rose AY, Song K, Chen Y, Bradley JM, Rookhuizen D, Acott TS
Investigative ophthalmology & visual science 2007 Jun;48(6):2634-43
Investigative ophthalmology & visual science 2007 Jun;48(6):2634-43
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- IL-1 RII Inhibition of IL-1 beta/IL-1F2-dependent Cell Proliferation and Neutralization by Human IL-1 RII Antibody. Recombinant Human IL-1 RII (Catalog # 263-2R) inhibits Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta/IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-263-NA). The ND50 is typically 15-30 µg/mL in the presence of concanavalin A (1.25 µg/mL).
