109-401-305

antibody from Rockland Immunochemicals, Inc.

Targeting: IL1R2 CD121b, IL1RB

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

109-401-305

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#109-401-305, RRID:AB_218928

Product name

Anti-Human IL-1 Receptor Type II (IL-1RII) (RABBIT) Antibody - 109-401-305

Antibody type

Polyclonal

Vial size

1 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's anti-IL-1RII antibody.  This blot shows detection of an IL-1RII-GST fusion protein (~500 ng, lane 1, green, 800 nm channel). Minimal reactivity is observed against GST (data not shown).  Protein was resolved on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.  After blocking, the membrane was probed with the primary antibody diluted to 1:1,000.  Incubation was for 2 hrs at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG (H&L) MX10 (611-132-122) for 45 min at room temperature.  Molecular weight markers are shown for size comparison (lane M, red, 700 nm channel).  IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.

Validation comment

Western Blot