Explore
Validate
Learn
Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00003663-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003663-M03, RRID:AB_539926
- Product name
- Mouse Monoclonal IRF5 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. IRF5 - interferon regulatory factor 5
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references IRF5 Is Required for Bacterial Clearance in Human M1-Polarized Macrophages, and IRF5 Immune-Mediated Disease Risk Variants Modulate This Outcome.
IRF5 and IRF5 Disease-Risk Variants Increase Glycolysis and Human M1 Macrophage Polarization by Regulating Proximal Signaling and Akt2 Activation.
Hedl M, Yan J, Witt H, Abraham C
Journal of immunology (Baltimore, Md. : 1950) 2019 Feb 1;202(3):920-930
Journal of immunology (Baltimore, Md. : 1950) 2019 Feb 1;202(3):920-930
IRF5 and IRF5 Disease-Risk Variants Increase Glycolysis and Human M1 Macrophage Polarization by Regulating Proximal Signaling and Akt2 Activation.
Hedl M, Yan J, Abraham C
Cell reports 2016 Aug 30;16(9):2442-55
Cell reports 2016 Aug 30;16(9):2442-55
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 expression in NIH/3T3 (Cat # L018V1).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 expression in A-431 (Cat # L015V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: IRF5 Antibody (1H6) [H00003663-M03] - Detection limit for recombinant GST tagged IRF5 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of monoclonal antibody to IRF5 on A-431 cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: IRF5 Antibody (1H6) [H00003663-M03] - Analysis of IRF5 transfected lysate using anti-IRF5 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRF5 mouse polyclonal antibody.
