- NB100-1092 - Novus Biologicals IRF5 antibody

Submitted references Specific detection of interferon regulatory factor 5 (IRF5): A case of antibody inequality.

Li D, De S, Li D, Song S, Matta B, Barnes BJ
Scientific reports 2016 Aug 2;6:31002

MyD88-dependent signaling influences fibrosis and alternative macrophage activation during Staphylococcus aureus biofilm infection.

Hanke ML, Angle A, Kielian T
PloS one 2012;7(8):e42476

Loss of interferon regulatory factor 5 (IRF5) expression in human ductal carcinoma correlates with disease stage and contributes to metastasis.

Bi X, Hameed M, Mirani N, Pimenta EM, Anari J, Barnes BJ
Breast cancer research : BCR 2011;13(6):R111

IRF5 is required for late-phase TNF secretion by human dendritic cells.

Krausgruber T, Saliba D, Ryzhakov G, Lanfrancotti A, Blazek K, Udalova IA
Blood 2010 Jun 3;115(22):4421-30

IRF-5 is a mediator of the death receptor-induced apoptotic signaling pathway.

Hu G, Barnes BJ
The Journal of biological chemistry 2009 Jan 30;284(5):2767-77

Methamphetamine enhances Hepatitis C virus replication in human hepatocytes.

Ye L, Peng JS, Wang X, Wang YJ, Luo GX, Ho WZ
Journal of viral hepatitis 2008 Apr;15(4):261-70

Three functional variants of IFN regulatory factor 5 (IRF5) define risk and protective haplotypes for human lupus.

Graham RR, Kyogoku C, Sigurdsson S, Vlasova IA, Davies LR, Baechler EC, Plenge RM, Koeuth T, Ortmann WA, Hom G, Bauer JW, Gillett C, Burtt N, Cunninghame Graham DS, Onofrio R, Petri M, Gunnarsson I, Svenungsson E, Rönnblom L, Nordmark G, Gregersen PK, Moser K, Gaffney PM, Criswell LA, Vyse TJ, Syvänen AC, Bohjanen PR, Daly MJ, Behrens TW, Altshuler D
Proceedings of the National Academy of Sciences of the United States of America 2007 Apr 17;104(16):6758-63

Interferon regulatory factor 5 represses expression of the Epstein-Barr virus oncoprotein LMP1: braking of the IRF7/LMP1 regulatory circuit.

Ning S, Huye LE, Pagano JS
Journal of virology 2005 Sep;79(18):11671-6

Virus-specific activation of a novel interferon regulatory factor, IRF-5, results in the induction of distinct interferon alpha genes.

Barnes BJ, Moore PA, Pitha PM
The Journal of biological chemistry 2001 Jun 29;276(26):23382-90

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: IRF5 Antibody [NB100-1092] - Human Spleen (A) and Peripheral Blood Lymphocytes (B) lysates (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: IRF5 Antibody [NB100-1092] - Comparative analysis of IRF5 protein expression between immortalized lymphoid cell lines by immunoblot. Endogenous IRF5 protein expression in lysates (T, THP-1; R, Ramos B cells; J, Jurkat T cells) was detecting by running multiple independent blots for the analysis of antibody specificity; seven different commercially available alpha-IRF5 antibodies were evaluated. IRF5 expression was detected with the previously validated Cell Signaling antibody #3257 that is no longer available. beta-actin levels and apparent molecular weight standards are shown as loading controls and for size comparison, respectively. Arrows indicate detection of an appropriately sized band(s) corresponding to IRF5. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep31002), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: IRF5 Antibody [NB100-1092] - Endogenous IRF5 knockdown analysis. Ramos B cells were nucleofected with scrambled (Scr) or IRF5 (KD) siRNAs and lysates from same nucleofection run on multiple independent blots for comparative analysis of seven different commercially available alpha-IRF5 antibodies. Arrows indicate detection of an appropriately sized band(s) corresponding to IRF5. Data are representative of three independent experiments. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep31002), licensed under a CC-BY licence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: IRF5 Antibody [NB100-1092] - Western blot analysis of Human Spleen (A) and A549 cell lysate (B) + peptide (C), 35 ug protein in RIPA buffer. Antibody at 1 ug/mL. Detected by chemiluminescence

NB100-1092

Antibody data