MA5-34689

antibody from Invitrogen Antibodies

Targeting: CKM CKMM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-34689

Provider

Invitrogen Antibodies

Product name

Creatine Kinase MB Recombinant Rabbit Monoclonal Antibody (JB78-34)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: Rat brain tissue, human prostate tissue, mouse colon tissue, mouse brain tissue lysate, 293, 293T, LOVO.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

JB78-34

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Creatine kinase B type in Lane 1: Mouse brain, Lane 2: Mouse brain, Lane 3: Rat brain, Lane 4: 293. Samples were incubated with Creatine kinase B type monoclonal antibody (Product # MA5-34689), at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Creatine kinase B type in Lane 1: Mouse brain, Lane 2: Mouse brain, Lane 3: Rat brain, Lane 4: 293. Samples were incubated with Creatine kinase B type monoclonal antibody (Product # MA5-34689), at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Creatine kinase B type in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Creatine kinase B type monoclonal antibody (Product # MA5-34689), followed by DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Creatine kinase B type in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Creatine kinase B type monoclonal antibody (Product # MA5-34689), followed by DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Creatine kinase B type in paraffin-embedded rat brain tissue. Samples were incubated with Creatine kinase B type monoclonal antibody (Product # MA5-34689), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Creatine kinase B type in paraffin-embedded mouse colon tissue. Samples were incubated with Creatine kinase B type monoclonal antibody (Product # MA5-34689), and followed by hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Creatine kinase B type in paraffin-embedded human prostate tissue. Samples were incubated with Creatine kinase B type monoclonal antibody (Product # MA5-34689), and followed by hematoxylin.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Creatine kinase B type in 293T cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Creatine kinase B type monoclonal antibody (Product # MA5-34689) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.