- GTX115378 - GeneTex CALCOCO2 antibody

Submitted references Dual Inhibition of PIK3C3 and FGFR as a New Therapeutic Approach to Treat Bladder Cancer.

Chen CH, Changou CA, Hsieh TH, Lee YC, Chu CY, Hsu KC, Wang HC, Lin YC, Lo YN, Liu YR, Liou JP, Yen Y
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Mar 1;24(5):1176-1189

Age-dependent changes in synaptic plasticity enhance tau oligomerization in the mouse hippocampus.

Kimura T, Suzuki M, Akagi T
Acta neuropathologica communications 2017 Sep 6;5(1):67

NDP52 activates nuclear myosin VI to enhance RNA polymerase II transcription.

Fili N, Hari-Gupta Y, Dos Santos Á, Cook A, Poland S, Ameer-Beg SM, Parsons M, Toseland CP
Nature communications 2017 Nov 30;8(1):1871

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Enhanced validation

Submitted by: GeneTex (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
KD Reagent Type: siRNA
Downregulation: <50%
KD Reagent Prod page: KD Reagent Prod page
KD Reagent Provider: shRNA reagents was provided courtesy of cooperative labs.
KD Reagent Prod no: Proprietary.
Antibody Lot Number: 40302

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ?g of whole cell lysate) A: Raji 10% SDS PAGE GTX115378 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NDP52 antibody detects NDP52 protein by Western blot analysis.A. 20 ?g Huh7 whole cell lysate/extract (untreated) B. 20 ?g Huh7 whole cell lysate/extract (3uM-Thapsipargin treatment for 12hr)0 % SDS-PAGENDP52 antibody (GTX115378) dilution: 1:1500
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) Huh-7 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NDP52 antibody detects NDP52 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12hrs and 24hrs) HepG2 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted by 1:2000.The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) HepG2 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with NDP52 antibody (GTX115378) diluted at 1:4000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescence analysis of methanol-fixed A431, using NDP52(GTX115378) antibody at 1:200 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: NDP52 antibody detects NDP52 protein at autophagosome by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 50£gM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: NDP52 protein stained by NDP52 antibody (GTX115378) diluted at 1:1000.Red: Phalloidin, a F-actin marker.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of NDP52 protein from Jurkat whole cell extracts using 5 £gg of NDP52 antibody (GTX115378).Western blot analysis was performed using NDP52 antibody (GTX115378).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded H441 xenograft, using NDP52(GTX115378) antibody at 1:100 dilution.

GTX115378

Antibody data