PA5-112332

antibody from Invitrogen Antibodies

Targeting: KAT7 HBO1, HBOA, MYST2, ZC2HC7

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-112332

Provider

Invitrogen Antibodies

Product name

MYST2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MYST2 using MYST2 Polyclonal Antibody (Product # PA5-112332) at a dilution of 1:500. Lane A: Jurkat whole cell lysate, Lane B: HeLa whole cell lysate, (Lysates/proteins at 30 ?g per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 75 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MYST2 using MYST2 Polyclonal Antibody (Product # PA5-112332) at a dilution of 1:500. Lane A: Jurkat whole cell lysate, Lane B: HeLa whole cell lysate, (Lysates/proteins at 30 µg per lane). The secondary antibody used was a Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 75 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MYST2 in PC3 cells. Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with MYST2 Polyclonal Antibody (Product # PA5-112332) (1:200) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MYST2 in formalin-fixed paraffin embedded sections human skin tissue sections using MYST2 Polyclonal Antibody (Product # PA5-112332) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MYST2 in formalin-fixed paraffin embedded sections human stomach tissue sections using MYST2 Polyclonal Antibody (Product # PA5-112332) at a dilution of 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation of MYST2 was performed on (Lane A) 0.5 mg Jurkat whole cell lysate using 4 µL of MYST2 Polyclonal Antibody (Product # PA5-112332) at a dilution of 1:100, and 60 µg of Immunomagnetic beads Protein A/G. A Goat Anti-Rabbit IgG (H+L)/HRP was used as a secondary antibody at a dilution of 1:10,000. Developed using the ECL technique and performed under reducing conditions. Predicted band size: 71 kDa. Observed band size: 90 kDa.