Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-19978 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MTA2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- In Western blot applications, this antibody detects a band at ~75kDa. A suggested positive control is Hela cell lysate.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PID expression in HeLa whole cell lysates in the absence (A) or presence (B) of blocking peptide with MTA2 Polyclonal Antibody (Product # PA5-19978) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PID expression in HeLa whole cell lysates in the absence (A) or presence (B) of blocking peptide with MTA2 Polyclonal Antibody (Product # PA5-19978) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of MTA2 was achieved by transfecting HeLa with MTA2 specific siRNAs (Silencer® select Products # s17390, s17389). Western blot analysis (Fig. a) was performed using whole cell Lysates from the MTA2 knockdown (siRNA treated) cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with MTA2 Polyclonal Antibody (Product # PA5-19978, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to MTA2. Few uncharacterized bands (*) were observed between 20-30kDa.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MTA2 Polyclonal Antibody (Product # PA5-19978) and ~75 kDa band corresponding to MTA2 was observed in all the cell lines and tissues tested along with an uncharacterized band (*) at ~20 and 30 kDa. Whole cell lysate (40ug lysate) of Raji (Lane 1), A-431 (Lane 2), NIH: OVCAR-3 (Lane 3), IMR-32 (Lane 4), BeWo (Lane 5), HeLa (Lane 6), Mouse Ovary (Lane 7) and Rat Ovary (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The bot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using a PID polyclonal antibody (Product # PA5-19978) at a 10 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of HeLa cells using MTA2 Polyclonal Antibody (Product # PA5-19978) at 10 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of PID in HeLa cells with MTA2 Polyclonal Antibody (Product # PA5-19978) at 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of PID in human small intestine tissue with MTA2 Polyclonal Antibody (Product # PA5-19978) at 2.5 µg/mL.