Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
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Validation data
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- Product number
- LS-C290534 - Provider product page
- Provider
- LSBio
- Product name
- EXOSC2 / RRP4 Antibody (aa243-293) LS-C290534
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2-8°C for up to one year.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human EXOSC2 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified rabbit anti-EXOSC2 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Mouse EXOSC2 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EXOSC2 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of human NAF1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg), HeLa (50µg), and Jurkat (50µg) cells. Antibodies: Affinity purified rabbit anti-NAF1 antibody used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Human EXOSC2 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 ug), HeLa (50 ug), and Jurkat (50 ug) cells. Antibodies: Affinity purified rabbit anti-EXOSC2 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Mouse EXOSC2 by Western Blot. Samples: Whole cell lysate (15 and 50 ug) from mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-EXOSC2 antibody used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of Human EXOSC2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-EXOSC2 antibody used for IP at 6 ug/mg lysate. EXOSC2 was also immunoprecipitated by rabbit anti-EXOSC2 antibody BL14001. For blotting immunoprecipitated EXOSC2, was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection of human NAF1 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg), HeLa (50µg), and Jurkat (50µg) cells. Antibodies: Affinity purified rabbit anti-NAF1 antibody used for WB at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.