H00004802-M01-50ug

antibody from Novus Biologicals

Targeting: NFYC CBF-C, NF-YC

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00004802-M01-50ug

Provider

Novus Biologicals

Product name

Mouse Monoclonal NFYC Antibody

Antibody type

Monoclonal

Description

IgG purified. NFYC - nuclear transcription factor Y, gamma

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

50 ug

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NFYC Antibody (1D3) [H00004802-M01] - Analysis of NFYC over-expressed 293 cell line, cotransfected with NFYC Validated Chimera RNAi ( Cat # H00004802-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYC monoclonal antibody (M01), clone 1D3 (Cat # H00004802-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NFYC Antibody (1D3) [H00004802-M01] - Analysis of NFYC expression in transfected 293T cell line by NFYC monoclonal antibody (M01), clone 1D3.Lane 1: NFYC transfected lysate(37.2 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

ELISA: NFYC Antibody (1D3) [H00004802-M01] - Detection limit for recombinant GST tagged NFYC is 3 ng/ml as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: NFYC Antibody (1D3) [H00004802-M01] - Analysis of monoclonal antibody to NFYC on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: NFYC Antibody (1D3) [H00004802-M01] - Analysis of monoclonal antibody to NFYC on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.