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Learn600-401-435
antibody from Rockland Immunochemicals, Inc.
Targeting: HIF3A
bHLHe17, IPAS, MOP7, PASD7
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 600-401-435 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-435, RRID:AB_2263987
- Product name
- Anti-Hif3a (RABBIT) Antibody - 600-401-435
- Antibody type
- Polyclonal
- Vial size
- 100 µl
Submitted references Placental but not heart defects are associated with elevated hypoxia-inducible factor alpha levels in mice lacking prolyl hydroxylase domain protein 2.
Takeda K, Ho VC, Takeda H, Duan LJ, Nagy A, Fong GH
Molecular and cellular biology 2006 Nov;26(22):8336-46
Molecular and cellular biology 2006 Nov;26(22):8336-46
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's Affinity Purified anti-Hif antibody shows detection of a band ~72 kDa corresponding to mouse Hif3a (arrowhead). Approximately 10 ?g of a CoCl2 treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 uM CoCl2 for 6 h at 37° C effectively mimics hypoxia. After blocking the membrane was probed overnight at 4° C with the primary antibody diluted to 1:1,600. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-132-122) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Validation comment
- Western Blot
