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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- PAB10271 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10271, RRID:AB_1674685
- Product name
- Hif3a polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Hif3a.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Structural basis for PAS domain heterodimerization in the basic helix--loop--helix-PAS transcription factor hypoxia-inducible factor.
Inhibitory PAS domain protein (IPAS) is a hypoxia-inducible splicing variant of the hypoxia-inducible factor-3alpha locus.
Cross-talk between the aryl hydrocarbon receptor and hypoxia inducible factor signaling pathways. Demonstration of competition and compensation.
Erbel PJ, Card PB, Karakuzu O, Bruick RK, Gardner KH
Proceedings of the National Academy of Sciences of the United States of America 2003 Dec 23;100(26):15504-9
Proceedings of the National Academy of Sciences of the United States of America 2003 Dec 23;100(26):15504-9
Inhibitory PAS domain protein (IPAS) is a hypoxia-inducible splicing variant of the hypoxia-inducible factor-3alpha locus.
Makino Y, Kanopka A, Wilson WJ, Tanaka H, Poellinger L
The Journal of biological chemistry 2002 Sep 6;277(36):32405-8
The Journal of biological chemistry 2002 Sep 6;277(36):32405-8
Cross-talk between the aryl hydrocarbon receptor and hypoxia inducible factor signaling pathways. Demonstration of competition and compensation.
Chan WK, Yao G, Gu YZ, Bradfield CA
The Journal of biological chemistry 1999 Apr 23;274(17):12115-23
The Journal of biological chemistry 1999 Apr 23;274(17):12115-23
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using Hif3a polyclonal antibody (Cat # PAB10271) shows detection of aband ~72 KDa corresponding to mouse Hif3a (arrowhead).Approximately 10 ug of a CoCl2 treated NIH/3T3 cell lysate (Lane 1) and control NIH/3T3 cell lysate (Lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.Treatment of exponentially growing NIH/3T3 cells with 130 uM CoCl2 for 6 h at 37°C effectively mimicshypoxia.After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:1,600.The membrane was washed and reacted with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature.IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
