R1575P

antibody from Acris Antibodies GmbH

Targeting: HIF3A bHLHe17, IPAS, MOP7, PASD7

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R1575P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1575P, RRID:AB_1002380

Product name

anti HIF3A / HIF3 alpha (581-592)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 581-592 of Mouse HIF3A.

Reactivity

Mouse, Rat

Host

Rabbit

Vial size

0.1 mg

Concentration

1.2 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Purified anti-Hif3 α antibody shows detection of a band ~72 kDa corresponding to mouse Hif3a (arrowhead). Approximately 10 µg of a CoCl2 treated 3T3 cell lysate (lane 1) and control 3T3 cell lysate (lane 2) were separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. Treatment of exponentially growing 3T3 cells with 130 µM CoCl2 for 6 h at 37°C effectively mimicshypoxia. After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:1,600. The membrane was washed and reacted with a 1:10,000 dilution of IRDyeTM 800 conjugated Gt-a-Rabbit IgG [H&L] for 45 min at room temperature. IRDyeTM 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Formalin-Fixed, Paraffin-Embedded human lung, respiratory epithelium tissue stained with HIF3A Antibody Cat.-No R1575P after heat-induced antigen retrieval.

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Formalin-Fixed, Paraffin-Embedded human Placenta tissue stained with HIF3A Antibody Cat.-No R1575P after heat-induced antigen retrieval.