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- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NBP1-03155 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-03155, RRID:AB_1521175
- Product name
- Rabbit Polyclonal HIF-3 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. No reactivity is expected against other forms of Hif proteins. Reactivity against homologues from other sources is not known.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1.0 mg/ml
- Storage
- Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Submitted references Hypoxia inducible factor 3α plays a critical role in alveolarization and distal epithelial cell differentiation during mouse lung development.
Huang Y, Kapere Ochieng J, Kempen MB, Munck AB, Swagemakers S, van Ijcken W, Grosveld F, Tibboel D, Rottier RJ
PloS one 2013;8(2):e57695
PloS one 2013;8(2):e57695
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HIF-3 alpha Antibody [NBP1-03155] - (lane 1) 3T3 cell lysate and (lane 2) control 3T3 cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: HIF-3 alpha Antibody [NBP1-03155] - IPF derived fibroblasts have low expression of HIF-3alpha by hypermethylation. Western blot of HIF3alpha in IPF derived fibroblasts after treatment with 2 and 5uM of 5-Aza-2'-deoxycytidine (5-Aza) for 5 days. beta-actin was used as a loading control. Image collected and cropped by CiteAb from the following publication (https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-019-1100-4), licensed under a CC-BY licence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: HIF-3 alpha Antibody [NBP1-03155] - Expression of hypoxia-inducible alpha subunits in normal and diseased lung tissue. There is no significant difference in HIF-3alpha expression in fibroblasts; however, in the alveolar wall, the latter shows a positive IHC reaction in different cellular elements in controls (i) and hypersensitivity pneumonitis (h), while no expression in idiopathic pulmonary fibrosis (g). Image collected and cropped by CiteAb from the following publication (https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-019-1100-4), licensed under a CC-BY licence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HIF-3 alpha Antibody [NBP1-03155] - Staining of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: HIF-3 alpha Antibody [NBP1-03155] - Staining of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
