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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00051728-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00051728-M01, RRID:AB_2268403
- Product name
- Mouse Monoclonal POLR3K Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. POLR3K - polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: POLR3K Antibody (3F5) [H00051728-M01] - Analysis of POLR3K expression in transfected 293T cell line by POLR3K monoclonal antibody (M01), clone 3F5.Lane 1: POLR3K transfected lysate(12.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: POLR3K Antibody (3F5) [H00051728-M01] - Detection limit for recombinant GST tagged POLR3K is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: POLR3K Antibody (3F5) [H00051728-M01] - Analysis of monoclonal antibody to POLR3K on HeLa cell . Antibody concentration 10 ug/ml.
