H00136319-M14

antibody from Abnova Corporation

Targeting: MTPN GCDP, MYOTROPHIN, V-1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00136319-M14

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00136319-M14, RRID:AB_1679423

Product name

MTPN monoclonal antibody (M14), clone 1F3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full-length recombinant MTPN.

Antigen sequence

MCDKEFMWALKNGGLDEVKDYVAKGEDVNRTLEGG
RKPLHYAADCGQLEILEFLLLKGADINAPDKHHIT
PLLSAVYEGHVSCVKLLLSKGADKTVKGPDGLTAF
EATDNQAIKALLQ


Isotype

IgG

Antibody clone number

1F3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MTPN expression in transfected 293T cell line by MTPN monoclonal antibody (M14), clone 1F3.Lane 1: MTPN transfected lysate(12.9 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged MTPN is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to MTPN on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol