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antibody from Invitrogen Antibodies
Targeting: HLTF
HIP116A, HLTF1, RNF80, SMARCA3, SNF2L3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-30173 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HLTF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa.
- Concentration
- 0.62 mg/mL
Submitted references Rad5 and Its Human Homologs, HLTF and SHPRH, Are Novel Interactors of Mismatch Repair.
Miller AK, Mao G, Knicely BG, Daniels HG, Rahal C, Putnam CD, Kolodner RD, Goellner EM
Frontiers in cell and developmental biology 2022;10:843121
Frontiers in cell and developmental biology 2022;10:843121
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HLTF Polyclonal Antibody (Product # PA5-30173). Sample (30 µg of whole cell lysate). Lane A: Hela . 7.5% SDS PAGE. HLTF Polyclonal Antibody (Product # PA5-30173) diluted at 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HLTF in paraformaldehyde-fixed HeLa cells using a HLTF polyclonal antibody (Product # PA5-30173) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MB157 xenograft, using HLTF (Product # PA5-30173) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HLTF antibody immunoprecipitates HLTF protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 µg HeLa whole cell lysate/extract B. Control with 2 µg of preimmune rabbit IgG C. Immunoprecipitation of HLTF protein by 2 µg of HLTF antibody (Product # PA5-30173) 7.5% SDS-PAGE The immunoprecipitated HLTF protein was detected by HLTF antibody (Product # PA5-30173) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 4 Human homologs of Rad5 HLTF and SHPRH interact with MSH2 and MLH1. (A) HeLa cells were treated with DMSO or 30 muM MNNG and lysates were fractionated to obtain the nuclear fraction. Nuclear fractions were immunoprecipitated with either anti-MLH1 or anti-MSH2 beads and immunoblotted for either HLTF and SHPRH. HLTF co-immunoprecipitated with MSH2. Immunoprecipitated HLTF runs at the predicted molecular weight of 116 kDa, however the non-immunoprecipitated HLTF in the input lanes runs at a slightly higher MW and has several additional bands consistent with the product sheet for the ThermoFisher HLTF antibody. SHPRH co-immunoprecipitated with MLH1 and the interaction is increased after MNNG treatment. (B) HeLa cell nuclear lysates were treated with or without DNAse. Nuclear fractions were obtained and immunoprecipitated with anti-HLTF beads and immunoblotted for MSH2. MSH2 interacts with HLTF regardless of DNAse treatment.
