Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF5726 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal TFCP2L1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human TFCP2L1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive Pluripotency.
Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys.
Transcription Factors Drive Tet2-Mediated Enhancer Demethylation to Reprogram Cell Fate.
Honey bee Royalactin unlocks conserved pluripotency pathway in mammals.
Trim33 regulates early maturation of mouse embryoid bodies in vitro.
α-Intercalated cells defend the urinary system from bacterial infection.
Bredenkamp N, Yang J, Clarke J, Stirparo GG, von Meyenn F, Dietmann S, Baker D, Drummond R, Ren Y, Li D, Wu C, Rostovskaya M, Eminli-Meissner S, Smith A, Guo G
Stem cell reports 2019 Dec 10;13(6):1083-1098
Stem cell reports 2019 Dec 10;13(6):1083-1098
Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys.
Kang Y, Ai Z, Duan K, Si C, Wang Y, Zheng Y, He J, Yin Y, Zhao S, Niu B, Zhu X, Liu L, Xiang L, Zhang L, Niu Y, Ji W, Li T
Cell reports 2018 Nov 27;25(9):2563-2576.e9
Cell reports 2018 Nov 27;25(9):2563-2576.e9
Transcription Factors Drive Tet2-Mediated Enhancer Demethylation to Reprogram Cell Fate.
Sardina JL, Collombet S, Tian TV, Gómez A, Di Stefano B, Berenguer C, Brumbaugh J, Stadhouders R, Segura-Morales C, Gut M, Gut IG, Heath S, Aranda S, Di Croce L, Hochedlinger K, Thieffry D, Graf T
Cell stem cell 2018 Nov 1;23(5):727-741.e9
Cell stem cell 2018 Nov 1;23(5):727-741.e9
Honey bee Royalactin unlocks conserved pluripotency pathway in mammals.
Wan DC, Morgan SL, Spencley AL, Mariano N, Chang EY, Shankar G, Luo Y, Li TH, Huh D, Huynh SK, Garcia JM, Dovey CM, Lumb J, Liu L, Brown KV, Bermudez A, Luong R, Zeng H, Mascetti VL, Pitteri SJ, Wang J, Tu H, Quarta M, Sebastiano V, Nusse R, Rando TA, Carette JE, Bazan JF, Wang KC
Nature communications 2018 Dec 4;9(1):5078
Nature communications 2018 Dec 4;9(1):5078
Trim33 regulates early maturation of mouse embryoid bodies in vitro.
Rajderkar S, Panaretos C, Kaartinen V
Biochemistry and biophysics reports 2017 Dec;12:185-192
Biochemistry and biophysics reports 2017 Dec;12:185-192
α-Intercalated cells defend the urinary system from bacterial infection.
Paragas N, Kulkarni R, Werth M, Schmidt-Ott KM, Forster C, Deng R, Zhang Q, Singer E, Klose AD, Shen TH, Francis KP, Ray S, Vijayakumar S, Seward S, Bovino ME, Xu K, Takabe Y, Amaral FE, Mohan S, Wax R, Corbin K, Sanna-Cherchi S, Mori K, Johnson L, Nickolas T, D'Agati V, Lin CS, Qiu A, Al-Awqati Q, Ratner AJ, Barasch J
The Journal of clinical investigation 2014 Jul;124(7):2963-76
The Journal of clinical investigation 2014 Jul;124(7):2963-76
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human TFCP2L1 by Western Blot. Western blot shows lysates of human placental tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TFCP2L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5726) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TFCP2L1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.