AF5726

antibody from R&D Systems

Targeting: TFCP2L1 CRTR1, LBP-9

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF5726

Provider

R&D Systems

Product name

Human TFCP2L1 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human TFCP2L1 in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

Q9NZI6


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Improving Cell Survival in Injected Embryos Allows Primed Pluripotent Stem Cells to Generate Chimeric Cynomolgus Monkeys.

Kang Y, Ai Z, Duan K, Si C, Wang Y, Zheng Y, He J, Yin Y, Zhao S, Niu B, Zhu X, Liu L, Xiang L, Zhang L, Niu Y, Ji W, Li T
Cell reports 2018 Nov 27;25(9):2563-2576.e9

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Transcription Factors Drive Tet2-Mediated Enhancer Demethylation to Reprogram Cell Fate.

Sardina JL, Collombet S, Tian TV, Gómez A, Di Stefano B, Berenguer C, Brumbaugh J, Stadhouders R, Segura-Morales C, Gut M, Gut IG, Heath S, Aranda S, Di Croce L, Hochedlinger K, Thieffry D, Graf T
Cell stem cell 2018 Nov 1;23(5):727-741.e9

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Honey bee Royalactin unlocks conserved pluripotency pathway in mammals.

Wan DC, Morgan SL, Spencley AL, Mariano N, Chang EY, Shankar G, Luo Y, Li TH, Huh D, Huynh SK, Garcia JM, Dovey CM, Lumb J, Liu L, Brown KV, Bermudez A, Luong R, Zeng H, Mascetti VL, Pitteri SJ, Wang J, Tu H, Quarta M, Sebastiano V, Nusse R, Rando TA, Carette JE, Bazan JF, Wang KC
Nature communications 2018 Dec 4;9(1):5078

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Trim33 regulates early maturation of mouse embryoid bodies in vitro.

Rajderkar S, Panaretos C, Kaartinen V
Biochemistry and biophysics reports 2017 Dec;12:185-192

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α-Intercalated cells defend the urinary system from bacterial infection.

Paragas N, Kulkarni R, Werth M, Schmidt-Ott KM, Forster C, Deng R, Zhang Q, Singer E, Klose AD, Shen TH, Francis KP, Ray S, Vijayakumar S, Seward S, Bovino ME, Xu K, Takabe Y, Amaral FE, Mohan S, Wax R, Corbin K, Sanna-Cherchi S, Mori K, Johnson L, Nickolas T, D'Agati V, Lin CS, Qiu A, Al-Awqati Q, Ratner AJ, Barasch J
The Journal of clinical investigation 2014 Jul;124(7):2963-76

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human TFCP2L1 by Western Blot. Western blot shows lysates of human placental tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TFCP2L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5726) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TFCP2L1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.