AP09130PU-N

antibody from Acris Antibodies GmbH

Targeting: CCNL1 ania-6a

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP09130PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09130PU-N, RRID:AB_2035391

Product name

anti Cyclin L1 (Isoform 1)

Antibody type

Polyclonal

Antigen

Synthetic polypeptide corresponding to amino acids 314-369 of Human Cyclin L1alpha protein

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.5 mg

Concentration

5.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using Protein A Purified anti-Cyclin L1alpha antibody shows detection of a band ~59 kDa corresponding to a Cyclin L1alpha (arrowhead) present in mouse brain whole cell lysate. Approximately 35 µg of lysate was separated by 4-20% SDS-PAGE followed by transfer to nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:3,500 for 2h at room temperature followed by washes and reaction with a 1:10,000 dilution of IRDye(TM)800 conjugated Gt-a-Rabbit IgG [H&L] MX for 45 min at room temperature. IRDye(TM)800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry. Anti-Cyclin L1alpha antibody was used at a 10 µg/ml to detect Cyclin L1alpha in a variety of tissues including breast (collagen), heart, kidney (distal tubules), liver, skeletal muscle, ovary (granulosa and oocyte), pancreas (islet), placenta (trophoblast), prostate (epithelium), skin, spleen (endothelium), stomach (chief), testes (seminiferous epithelium and leydig), thymus (Hassals corpuscle and lymphocytes) and uterus (glandular epithelium and stroma). Low to moderate background staining was noted. This image shows Cyclin L1alpha staining of human spleen. Tissue was formalin-fixed and paraffin embedded.