Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- MA5-32355 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- pro-Caspase 7 Recombinant Rabbit Monoclonal Antibody (SD20-57)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- SD20-57
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of pro-Caspase 7 in human lung cell lysate using a pro-Caspase 7 Monoclonal antibody (Product # MA5-32355) at a dilution of 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-pro-Caspase 7 Recombinant Rabbit Monoclonal Antibody (SD20-57) (Product # MA5-32355) and a 35kDa band corresponding to Caspase-7 was observed across cell lines tested and decreased upon Etoposide and Staurosporine treatment due to cleavage of the full length Caspase-7. Whole cell extracts (30 µg lysate) of Jurkat (Lane 1), Jurkat treated with Etoposide (25uM for 16hr) (Lane 2), HeLa (Lane 3) and HeLa treated with Staurosporine (1uM for 5hr) (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of pro-Caspase 7 in HepG2 cells using a pro-Caspase 7 Monoclonal antibody (Product # MA5-32355) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of pro-Caspase 7 in Hela cells using a pro-Caspase 7 Monoclonal antibody (Product # MA5-32355) as seen in green. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of pro-Caspase 7 of paraffin-embedded Human liver tissue using a pro-Caspase-7 Monoclonal antibody (Product #MA5-32355). Counter stained with hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of pro-Caspase 7 of paraffin-embedded Human kidney tissue using a pro-Caspase-7 Monoclonal antibody (Product #MA5-32355). Counter stained with hematoxylin.