PAB12874

antibody from Abnova Corporation

Targeting: CARD9

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB12874

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB12874, RRID:AB_10550677

Product name

CARD9 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of CARD9.

Antigen sequence

DRENTTGSDNTDTEGS


Storage

Store at 4°C for three months. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Impaired RASGRF1/ERK-mediated GM-CSF response characterizes CARD9 deficiency in French-Canadians.

Gavino C, Hamel N, Zeng JB, Legault C, Guiot MC, Chankowsky J, Lejtenyi D, Lemire M, Alarie I, Dufresne S, Boursiquot JN, McIntosh F, Langelier M, Behr MA, Sheppard DC, Foulkes WD, Vinh DC
The Journal of allergy and clinical immunology 2016 Apr;137(4):1178-1188.e7

---

CARD9 is a novel caspase recruitment domain-containing protein that interacts with BCL10/CLAP and activates NF-kappa B.

Bertin J, Guo Y, Wang L, Srinivasula SM, Jacobson MD, Poyet JL, Merriam S, Du MQ, Dyer MJ, Robison KE, DiStefano PS, Alnemri ES
The Journal of biological chemistry 2000 Dec 29;275(52):41082-6

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 polyclonal antibody (Cat # PAB12874) at 2.5 ug /mL .

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunocytochemistry of CARD9 in K-562 cells with CARD9 polyclonal antibody (Cat # PAB12874) at 10 ug/mL .

Validation comment

Immunocytochemistry