PA5-19993

antibody from Invitrogen Antibodies

Targeting: CARD9

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-19993

Provider

Invitrogen Antibodies

Product name

CARD9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

In Western blot applications, this antibody detects a band at ~59kDa. A suggested positive control is PC-3 cell lysate.

Concentration

1 mg/mL

References

Submitted references

Structures of autoinhibited and polymerized forms of CARD9 reveal mechanisms of CARD9 and CARD11 activation.

Holliday MJ, Witt A, Rodríguez Gama A, Walters BT, Arthur CP, Halfmann R, Rohou A, Dueber EC, Fairbrother WJ
Nature communications 2019 Jul 11;10(1):3070

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human MDA-MB-361 (A) and PC-3 (B) cell lysate using a CARD9 polyclonal antibody (Product # PA5-19993) at 2.5 µg /mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of CARD9 expression in human MDA-MB-361 (A) and PC-3 (B) cell lysate with CARD9 Polyclonal Antibody (Product # PA5-19993) at 2.5 µg/mL.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry of CARD9 in K562 cells with CARD9 Polyclonal Antibody (Product # PA5-19993) at 10 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry staining of K562 cells using a CARD9 polyclonal antibody (Product # PA5-19993) at a 10 µg/mL dilution.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 3 The CARD11 CARD-coiled-coil interface is weaker than that of CARD9, but is reinforced by the ID. a Cartoon schematic of the domain architecture of CARD9, CARD11-DeltaID, and CARD11 along with the chimeric constructs CARD9 1-131 /CARD11-DeltaID and CARD11 1-143 /CARD9, wherein the N-terminal residues comprising the CARD, linker, and CARD-interacting stretch of the coil-coil are swapped between the two proteins (See Supplementary Fig. 1B ). b Normalized NF-kappaB activation in HEK-293 cells upon overexpression of the indicated constructs. Three biologically independent experiments were undertaken, the outcomes of which are shown as dots. The mean value for each construct is depicted as a gray bar. Dotted line represents the value of the CARD9 WT construct, to which the other values were normalized. Representative western blot is shown for CARD9 and CARD11 constructs, using antibodies specific for regions outside of the N-terminal swapped domain or ID. Source data are provided in Source Data file