Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27170 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CXCR5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: HepG2. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CXCR5 Polyclonal Antibody (Product # PA5-27170). Sample (30 µg of whole cell lysate). Lane A: Hep G2. 10% SDS PAGE. CXCR5 Polyclonal Antibody (Product # PA5-27170) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCR5 Polyclonal Antibody detects CXCR5 protein at cell membrane by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CXCR5 stained by CXCR5 Polyclonal Antibody (Product # PA5-27170) diluted at 1:500. Blue: Fluoroshield with DAPI .
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCR5 Polyclonal Antibody detects CXCR5 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. CXCR5 stained by CXCR5 Polyclonal Antibody (Product # PA5-27170) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCR5 Polyclonal Antibody detects CXCR5 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human kidney. CXCR5 stained by CXCR5 Polyclonal Antibody (Product # PA5-27170) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CXCR5 Polyclonal Antibody detects CXCR5 protein at membrane on PC13 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded PC13 xenograft. CXCR5 Polyclonal Antibody (Product # PA5-27170) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.