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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-29845 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TBX5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa nuclear extract, rat brain. Predicted reactivity: Mouse (100%), Rat (99%), Japanese Medaka (94%), Xenopus laevis (98%), Chicken (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.31 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Physical stimulation by REAC and BMP4/WNT-1 inhibitor synergistically enhance cardiogenic commitment in iPSCs.
The Epigenetic Regulator HDAC1 Modulates Transcription of a Core Cardiogenic Program in Human Cardiac Mesenchymal Stromal Cells Through a p53-Dependent Mechanism.
Basoli V, Santaniello S, Rinaldi S, Fontani V, Pigliaru G, Wieser M, Strajeriu A, Castagna A, Redl H, Ventura C, Grillari R, Maioli M
PloS one 2019;14(1):e0211188
PloS one 2019;14(1):e0211188
The Epigenetic Regulator HDAC1 Modulates Transcription of a Core Cardiogenic Program in Human Cardiac Mesenchymal Stromal Cells Through a p53-Dependent Mechanism.
Moore JB 4th, Zhao J, Keith MC, Amraotkar AR, Wysoczynski M, Hong KU, Bolli R
Stem cells (Dayton, Ohio) 2016 Dec;34(12):2916-2929
Stem cells (Dayton, Ohio) 2016 Dec;34(12):2916-2929
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TBX5 using 30 µg of HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a TBX5 polyclonal antibody (Product # PA5-29845) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TBX5 was performed by separating 30 µg of HeLa whole cell and nuclear extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TBX5 Polyclonal Antibody (Product # PA5-29845) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TBX5 Polyclonal Antibody detects TBX5 protein by western blot analysis. A. 50 µg rat brain lysate/extract.7.5% SDS-PAGE. TBX5 Polyclonal Antibody (Product # PA5-29845) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TBX5 in paraformaldehyde-fixed HeLa cells using a TBX5 polyclonal antibody (Product # PA5-29845) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TBX5 Polyclonal Antibody detects TBX5 protein at nucleus on rat thyroid gland by immunohistochemical analysis. Sample: Paraffin-embedded rat thyroid gland. TBX5 Polyclonal Antibody (Product # PA5-29845) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- TBX5 Polyclonal Antibody detects TBX5 protein at nucleus on rat thyroid gland by immunohistochemical analysis. Sample: Paraffin-embedded rat thyroid gland. TBX5 Polyclonal Antibody (Product # PA5-29845) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
