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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NBP2-20713 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal TRIM29 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TRIM29 Antibody [NBP2-20713] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:2000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: TRIM29 Antibody [NBP2-20713] - A431 whole cell lysate/extract A : 30 ug whole cell lysate/extract of TRIM29 protein expressing A431 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of TRIM29 by 2.5 ug of TRIM29 antibody 10% SDS-PAGE The immunoprecipitated TRIM29 protein was detected by TRIM29 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TRIM29 Antibody [NBP2-20713] - MCF7 xenograft, using TRIM29 antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TRIM29 Antibody [NBP2-20713] - Mouse prostate. TRIM29 antibody [C1C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
