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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 600-401-B16S - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-B16S, RRID:AB_2062944
- Product name
- Anti-ATDC Ac-K116 (RABBIT) Antibody - 600-401-B16S
- Antibody type
- Polyclonal
- Vial size
- 25 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's affinity purified anti-ATDC (Ac-K116) antibody shows detection of a 66 kDa band corresponding to over-expressed, acetylated lysine (K116) ATDC (arrowhead) in transfected 293T cells. No staining is noted for cells transfected with empty vector only. No staining is noted for cells transfected with an ATDC K116R mutant (K to R transversion lacks site for acetylation). In each instance, samples were prepared with and without TSA (1.3uM, 6 hr) which inhibits deacetylation. Personal communication, Z. Yuan, H Lee Moffitt Cancer Center and Research Institute.
- Validation comment
- Western Blot
