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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-27642 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SAR1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431.
- Concentration
- 1 mg/mL
Submitted references The ULK1-FBXW5-SEC23B nexus controls autophagy.
Jeong YT, Simoneschi D, Keegan S, Melville D, Adler NS, Saraf A, Florens L, Washburn MP, Cavasotto CN, Fenyö D, Cuervo AM, Rossi M, Pagano M
eLife 2018 Dec 31;7
eLife 2018 Dec 31;7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SAR1A Polyclonal Antibody (Product # PA5-27642). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. 12% SDS PAGE. SAR1A Polyclonal Antibody (Product # PA5-27642) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SAR1A in methanol-fixed A431 cells using a SAR1A polyclonal antibody (Product # PA5-27642) at a 1:500 dilution.
