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Western blot
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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Immunocytochemistry [1]
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- Product number
- BML-SA475-0025 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#BML-SA475-0025, RRID:AB_2050587
- Product name
- 14-3-3_ (CT) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Rabbit, Sheep
- Host
- Rabbit
- Vial size
- 25 μl
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein.
Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1.
Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain.
Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants.
Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease.
14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain.
Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation.
Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins.
Expression and structural analysis of 14-3-3 proteins.
Purification of 14-3-3 protein and analysis of isoforms in chicken brain.
Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies.
Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.
Steinacker P, Schwarz P, Reim K, Brechlin P, Jahn O, Kratzin H, Aitken A, Wiltfang J, Aguzzi A, Bahn E, Baxter HC, Brose N, Otto M
Molecular and cellular biology 2005 Feb;25(4):1339-46
Molecular and cellular biology 2005 Feb;25(4):1339-46
Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1.
Chen HK, Fernandez-Funez P, Acevedo SF, Lam YC, Kaytor MD, Fernandez MH, Aitken A, Skoulakis EM, Orr HT, Botas J, Zoghbi HY
Cell 2003 May 16;113(4):457-68
Cell 2003 May 16;113(4):457-68
Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain.
Baxter HC, Liu WG, Forster JL, Aitken A, Fraser JR
Neuroscience 2002;109(1):5-14
Neuroscience 2002;109(1):5-14
Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants.
Aitken A
Plant molecular biology 2002 Dec;50(6):993-1010
Plant molecular biology 2002 Dec;50(6):993-1010
Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease.
Wiltfang J, Otto M, Baxter HC, Bodemer M, Steinacker P, Bahn E, Zerr I, Kornhuber J, Kretzschmar HA, Poser S, Rüther E, Aitken A
Journal of neurochemistry 1999 Dec;73(6):2485-90
Journal of neurochemistry 1999 Dec;73(6):2485-90
14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain.
Matto-Yelin M, Aitken A, Ravid S
Molecular biology of the cell 1997 Oct;8(10):1889-99
Molecular biology of the cell 1997 Oct;8(10):1889-99
Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation.
Wheeler-Jones CP, Learmonth MP, Martin H, Aitken A
The Biochemical journal 1996 Apr 1;315 ( Pt 1):41-7
The Biochemical journal 1996 Apr 1;315 ( Pt 1):41-7
Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins.
Jones DH, Ley S, Aitken A
FEBS letters 1995 Jul 10;368(1):55-8
FEBS letters 1995 Jul 10;368(1):55-8
Expression and structural analysis of 14-3-3 proteins.
Jones DH, Martin H, Madrazo J, Robinson KA, Nielsen P, Roseboom PH, Patel Y, Howell SA, Aitken A
Journal of molecular biology 1995 Jan 27;245(4):375-84
Journal of molecular biology 1995 Jan 27;245(4):375-84
Purification of 14-3-3 protein and analysis of isoforms in chicken brain.
Patel Y, Martin H, Howell S, Jones D, Robinson K, Aitken A
Biochimica et biophysica acta 1994 Jul 21;1222(3):405-9
Biochimica et biophysica acta 1994 Jul 21;1222(3):405-9
Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies.
Martin H, Rostas J, Patel Y, Aitken A
Journal of neurochemistry 1994 Dec;63(6):2259-65
Journal of neurochemistry 1994 Dec;63(6):2259-65
Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.
Martin H, Patel Y, Jones D, Howell S, Robinson K, Aitken A
FEBS letters 1993 Oct 4;331(3):296-303
FEBS letters 1993 Oct 4;331(3):296-303
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Western blot analysis of cell lysates using 14-3-3 _, pAb (Prod. No. BML-SA475). Antibody dilution 1:3'000, blot was developed by HRP/ECL.1. HeLa S100 (cytosol)2. HeLa (whole cell)3. Jurkat 4. MDCK 5. A4316. MDF-77. rat brain 8. PC12
