BML-SA475-0100

antibody from Enzo Life Sciences

Targeting: YWHAE FLJ45465

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

BML-SA475-0100

Provider

Enzo Life Sciences

Product name

14-3-3_ (CT) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat, Bovine, Chicken/Avian, Rabbit, Sheep

Host

Rabbit

Vial size

100 μl

Storage

-20°C

Handling

Avoid freeze/thaw cycles.

References

Submitted references

Unchanged survival rates of 14-3-3gamma knockout mice after inoculation with pathological prion protein.

Steinacker P, Schwarz P, Reim K, Brechlin P, Jahn O, Kratzin H, Aitken A, Wiltfang J, Aguzzi A, Bahn E, Baxter HC, Brose N, Otto M
Molecular and cellular biology 2005 Feb;25(4):1339-46

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Interaction of Akt-phosphorylated ataxin-1 with 14-3-3 mediates neurodegeneration in spinocerebellar ataxia type 1.

Chen HK, Fernandez-Funez P, Acevedo SF, Lam YC, Kaytor MD, Fernandez MH, Aitken A, Skoulakis EM, Orr HT, Botas J, Zoghbi HY
Cell 2003 May 16;113(4):457-68

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Immunolocalisation of 14-3-3 isoforms in normal and scrapie-infected murine brain.

Baxter HC, Liu WG, Forster JL, Aitken A, Fraser JR
Neuroscience 2002;109(1):5-14

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Functional specificity in 14-3-3 isoform interactions through dimer formation and phosphorylation. Chromosome location of mammalian isoforms and variants.

Aitken A
Plant molecular biology 2002 Dec;50(6):993-1010

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Isoform pattern of 14-3-3 proteins in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease.

Wiltfang J, Otto M, Baxter HC, Bodemer M, Steinacker P, Bahn E, Zerr I, Kornhuber J, Kretzschmar HA, Poser S, Rüther E, Aitken A
Journal of neurochemistry 1999 Dec;73(6):2485-90

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14-3-3 inhibits the Dictyostelium myosin II heavy-chain-specific protein kinase C activity by a direct interaction: identification of the 14-3-3 binding domain.

Matto-Yelin M, Aitken A, Ravid S
Molecular biology of the cell 1997 Oct;8(10):1889-99

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Identification of 14-3-3 proteins in human platelets: effects of synthetic peptides on protein kinase C activation.

Wheeler-Jones CP, Learmonth MP, Martin H, Aitken A
The Biochemical journal 1996 Apr 1;315 ( Pt 1):41-7

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Isoforms of 14-3-3 protein can form homo- and heterodimers in vivo and in vitro: implications for function as adapter proteins.

Jones DH, Ley S, Aitken A
FEBS letters 1995 Jul 10;368(1):55-8

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Expression and structural analysis of 14-3-3 proteins.

Jones DH, Martin H, Madrazo J, Robinson KA, Nielsen P, Roseboom PH, Patel Y, Howell SA, Aitken A
Journal of molecular biology 1995 Jan 27;245(4):375-84

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Purification of 14-3-3 protein and analysis of isoforms in chicken brain.

Patel Y, Martin H, Howell S, Jones D, Robinson K, Aitken A
Biochimica et biophysica acta 1994 Jul 21;1222(3):405-9

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Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies.

Martin H, Rostas J, Patel Y, Aitken A
Journal of neurochemistry 1994 Dec;63(6):2259-65

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Antibodies against the major brain isoforms of 14-3-3 protein. An antibody specific for the N-acetylated amino-terminus of a protein.

Martin H, Patel Y, Jones D, Howell S, Robinson K, Aitken A
FEBS letters 1993 Oct 4;331(3):296-303

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

Western blot analysis of cell lysates using 14-3-3 _, pAb (Prod. No. BML-SA475). Antibody dilution 1:3'000, blot was developed by HRP/ECL.1. HeLa S100 (cytosol)2. HeLa (whole cell)3. Jurkat 4. MDCK 5. A4316. MDF-77. rat brain 8. PC12