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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP54606PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP54606PU-N, RRID:AB_11142923
- Product name
- anti 14-3-3 protein epsilon (C-term)
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 229-259 amino acids from the C-terminal region of human YWHAE
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.25 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- YWHAE Antibody (C-term) (AP54606PU-N) western blot analysis in A375 cell line lysates (35 µg/lane). This demonstrates the YWHAE antibody detected the YWHAE protein (arrow).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- YWHAE Antibody (C-term) (AP54606PU-N) western blot analysis in mouse cerebellum tissue lysates (35 µg/lane). This demonstrates the YWHAE antibody detected the YWHAE protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- YWHAE Antibody (C-term) (AP54606PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the YWHAE Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- YWHAE Antibody (C-term) (AP54606PU-N) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
