TA309819

antibody from OriGene

Targeting: PRDM1 BLIMP1, PRDI-BF1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA309819

Provider

OriGene

Product name

Mouse Monoclonal Blimp-1 Antibody (3H2-E8)

Antibody type

Monoclonal

Description

Mouse Monoclonal Blimp-1 Antibody (3H2-E8)

Host

Mouse

Conjugate

Unconjugated

Epitope

PRDM1

Isotype

IgG

Antibody clone number

3H2-E8

Vial size

100 µl

Concentration

1.2mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot: Blimp-1 antibody - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using TA309794. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA Savitsky, Columbia University.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunoflourescence: Blimp-1 antibody - Double IF for Blimp-1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10um). Positive surface epithelium and centrocytes are labelled. Photo courtesy of Dr. Giorgio Cattoretti, Institute for Cancer Genetics, Columbia University, NY.

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometry / FACS analysis: Blimp-1 Antibody (3H2-E8) - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice (Tedder Lab; Duke Univ)

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometry / FACS analysis: Blimp-1 Antibody (3H2-E8) - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells (Tedder Lab; Duke Univ)

Validation comment

FC