600-401-B52

antibody from Rockland Immunochemicals, Inc.

Targeting: PRDM1 BLIMP1, PRDI-BF1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-B52

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#600-401-B52, RRID:AB_1961755

Product name

Anti-PRDM1/BLIMP1 (RABBIT) Antibody - 600-401-B52

Antibody type

Polyclonal

Vial size

100 µl

References

Submitted references

PD-1 Expression during Acute Infection Is Repressed through an LSD1-Blimp-1 Axis.

Bally APR, Neeld DK, Lu P, Majumder P, Tang Y, Barwick BG, Wang Q, Boss JM
Journal of immunology (Baltimore, Md. : 1950) 2020 Jan 15;204(2):449-458

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Blimp-1 represses CD8 T cell expression of PD-1 using a feed-forward transcriptional circuit during acute viral infection.

Lu P, Youngblood BA, Austin JW, Mohammed AU, Butler R, Ahmed R, Boss JM
The Journal of experimental medicine 2014 Mar 10;211(3):515-27

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blots using Rockland's affinity purified anti-PRDM1/BLIMP1 antibody show detection of overexpressed PRDM1/BLIMP1 in whole transfected Raji cell lysate (lane 2) at ~88kDa. Lane 1 shows mock transfection in whole Raji cell lysate. Detection of endogenous PRDM1/BLIMP1 (lane 4) is illustrated in human plasma cell nuclear extract, but not in Raji whole cell nuclear extract (lane 3). The identity of the lower dark band at ~50-60kDa is unknown. Primary antibody was used at a 1:1000 dilution in 5% PBS-Tween. Personal communication, Hyesuk Yoon and Jerry Boss, Emory University, Atlanta, GA

Validation comment

Western Blot