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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- MAB2327 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB2327, RRID:AB_10629245
- Product name
- Prdm1 monoclonal antibody, clone 3H2-E8
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant Prdm1.
- Isotype
- IgG
- Antibody clone number
- 3H2-E8
- Storage
- Store at 4°C for short term. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cytokine-dependent Blimp-1 expression in activated T cells inhibits IL-2 production.
Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice.
Toll-like receptor-dependent and -independent viperin gene expression and counter-regulation by PRDI-binding factor-1/BLIMP1.
PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage.
The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development.
Gong D, Malek TR
Journal of immunology (Baltimore, Md. : 1950) 2007 Jan 1;178(1):242-52
Journal of immunology (Baltimore, Md. : 1950) 2007 Jan 1;178(1):242-52
Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice.
Robertson EJ, Charatsi I, Joyner CJ, Koonce CH, Morgan M, Islam A, Paterson C, Lejsek E, Arnold SJ, Kallies A, Nutt SL, Bikoff EK
Development (Cambridge, England) 2007 Dec;134(24):4335-45
Development (Cambridge, England) 2007 Dec;134(24):4335-45
Toll-like receptor-dependent and -independent viperin gene expression and counter-regulation by PRDI-binding factor-1/BLIMP1.
Severa M, Coccia EM, Fitzgerald KA
The Journal of biological chemistry 2006 Sep 8;281(36):26188-95
The Journal of biological chemistry 2006 Sep 8;281(36):26188-95
PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage.
Cattoretti G, Angelin-Duclos C, Shaknovich R, Zhou H, Wang D, Alobeid B
The Journal of pathology 2005 May;206(1):76-86
The Journal of pathology 2005 May;206(1):76-86
The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development.
Chang DH, Cattoretti G, Calame KL
Mechanisms of development 2002 Sep;117(1-2):305-9
Mechanisms of development 2002 Sep;117(1-2):305-9
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection of Prdm1 in murine plasmacytoma cell lysate (P3X) using Prdm1 monoclonal antibody, clone 3H2-E8 (Cat # MAB2327).Murine pre-B cell lysate (negative control).Photo courtesy of DA Savitsky, Columbia University.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Double IF for Prdm1 (green) and Ki-67 (proliferation, red) with DAPI counterstain, at 10x magnification (scale bar is 10 um).Positive surface epithelium and centrocytes are labelled.Photo courtesy of Dr. Giorgio Cattoretti, Institute for Cancer Genetics, Columbia University, NY.
- Validation comment
- Immunofluorescence
