NB600-235

antibody from Novus Biologicals

Targeting: PRDM1 BLIMP1, PRDI-BF1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NB600-235

Provider

Novus Biologicals

Proper citation

Novus Cat#NB600-235, RRID:AB_10595225

Product name

Mouse Monoclonal BLIMP1/PRDM1 Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Mouse, Porcine

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Concentration

1 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

References

Submitted references

Extraoral plasmablastic lymphomas in a high human immunodeficiency virus endemic area.

Meer S, Perner Y, McAlpine ED, Willem P
Histopathology 2020 Jan;76(2):212-221

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ER stress-induced mediator C/EBP homologous protein thwarts effector T cell activity in tumors through T-bet repression.

Cao Y, Trillo-Tinoco J, Sierra RA, Anadon C, Dai W, Mohamed E, Cen L, Costich TL, Magliocco A, Marchion D, Klar R, Michel S, Jaschinski F, Reich RR, Mehrotra S, Cubillos-Ruiz JR, Munn DH, Conejo-Garcia JR, Rodriguez PC
Nature communications 2019 Mar 20;10(1):1280

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Molecular Signatures of Dengue Virus-Specific IL-10/IFN-γ Co-producing CD4 T Cells and Their Association with Dengue Disease.

Tian Y, Seumois G, De-Oliveira-Pinto LM, Mateus J, Herrera-de la Mata S, Kim C, Hinz D, Goonawardhana NDS, de Silva AD, Premawansa S, Premawansa G, Wijewickrama A, Balmaseda A, Grifoni A, Vijayanand P, Harris E, Peters B, Sette A, Weiskopf D
Cell reports 2019 Dec 24;29(13):4482-4495.e4

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Iguratimod represses B cell terminal differentiation linked with the inhibition of PKC/EGR1 axis.

Ye Y, Liu M, Tang L, Du F, Liu Y, Hao P, Fu Q, Guo Q, Yan Q, Zhang X, Bao C
Arthritis research & therapy 2019 Apr 11;21(1):92

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Epstein-Barr virus (EBV) provides survival factors to EBV⁺ diffuse large B-cell lymphoma (DLBCL) lines and modulates cytokine induced specific chemotaxis in EBV⁺  DLBCL.

Wu L, Ehlin-Henriksson B, Zhou X, Zhu H, Ernberg I, Kis LL, Klein G
Immunology 2017 Dec;152(4):562-573

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Therapeutically targeting mitochondrial redox signalling alleviates endothelial dysfunction in preeclampsia.

McCarthy C, Kenny LC
Scientific reports 2016 Sep 8;6:32683

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Rho kinase mediates right ventricular systolic dysfunction in rats with chronic neonatal pulmonary hypertension.

Gosal K, Dunlop K, Dhaliwal R, Ivanovska J, Kantores C, Desjardins JF, Connelly KA, McNamara PJ, Jain A, Jankov RP
American journal of respiratory cell and molecular biology 2015 Jun;52(6):717-27

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Mitochondrial function provides instructive signals for activation-induced B-cell fates.

Jang KJ, Mano H, Aoki K, Hayashi T, Muto A, Nambu Y, Takahashi K, Itoh K, Taketani S, Nutt SL, Igarashi K, Shimizu A, Sugai M
Nature communications 2015 Apr 10;6:6750

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The MEC1 and MEC2 lines represent two CLL subclones in different stages of progression towards prolymphocytic leukemia.

Rasul E, Salamon D, Nagy N, Leveau B, Banati F, Szenthe K, Koroknai A, Minarovits J, Klein G, Klein E
PloS one 2014;9(8):e106008

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C-type lectin-like receptor LOX-1 promotes dendritic cell-mediated class-switched B cell responses.

Joo H, Li D, Dullaers M, Kim TW, Duluc D, Upchurch K, Xue Y, Zurawski S, Le Grand R, Liu YJ, Kuroda M, Zurawski G, Oh S
Immunity 2014 Oct 16;41(4):592-604

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DRE-1/FBXO11-dependent degradation of BLMP-1/BLIMP-1 governs C. elegans developmental timing and maturation.

Horn M, Geisen C, Cermak L, Becker B, Nakamura S, Klein C, Pagano M, Antebi A
Developmental cell 2014 Mar 31;28(6):697-710

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p53 Induces skin aging by depleting Blimp1+ sebaceous gland cells.

Kim J, Nakasaki M, Todorova D, Lake B, Yuan CY, Jamora C, Xu Y
Cell death & disease 2014 Mar 27;5:e1141

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The transcriptional repressor BLIMP1 curbs host defenses by suppressing expression of the chemokine CCL8.

Severa M, Islam SA, Waggoner SN, Jiang Z, Kim ND, Ryan G, Kurt-Jones E, Charo I, Caffrey DR, Boyartchuk VL, Luster AD, Fitzgerald KA
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 1;192(5):2291-304

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Bach2 regulates homeostasis of Foxp3+ regulatory T cells and protects against fatal lung disease in mice.

Kim EH, Gasper DJ, Lee SH, Plisch EH, Svaren J, Suresh M
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 1;192(3):985-95

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The specialized proresolving mediator 17-HDHA enhances the antibody-mediated immune response against influenza virus: a new class of adjuvant?

Ramon S, Baker SF, Sahler JM, Kim N, Feldsott EA, Serhan CN, Martínez-Sobrido L, Topham DJ, Phipps RP
Journal of immunology (Baltimore, Md. : 1950) 2014 Dec 15;193(12):6031-40

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T cell-specific BLIMP-1 deficiency exacerbates experimental autoimmune encephalomyelitis in nonobese diabetic mice by increasing Th1 and Th17 cells.

Lin MH, Yeh LT, Chen SJ, Chiou HY, Chu CC, Yen LB, Lin KI, Chang DM, Sytwu HK
Clinical immunology (Orlando, Fla.) 2014 Apr;151(2):101-13

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IFN-α regulates Blimp-1 expression via miR-23a and miR-125b in both monocytes-derived DC and pDC.

Parlato S, Bruni R, Fragapane P, Salerno D, Marcantonio C, Borghi P, Tataseo P, Ciccaglione AR, Presutti C, Romagnoli G, Bozzoni I, Belardelli F, Gabriele L
PloS one 2013;8(8):e72833

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Upregulated hPuf-A promotes breast cancer tumorigenesis.

Fan CC, Lee LY, Yu MY, Tzen CY, Chou C, Chang MS
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine 2013 Oct;34(5):2557-64

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The product of Kaposi's sarcoma-associated herpesvirus immediate early gene K4.2 regulates immunoglobulin secretion and calcium homeostasis by interacting with and inhibiting pERP1.

Wong LY, Brulois K, Toth Z, Inn KS, Lee SH, O'Brien K, Lee H, Gao SJ, Cesarman E, Ensser A, Jung JU
Journal of virology 2013 Nov;87(22):12069-79

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B lymphocyte-induced maturation protein 1 (BLIMP-1) attenuates autoimmune diabetes in NOD mice by suppressing Th1 and Th17 cells.

Lin MH, Chou FC, Yeh LT, Fu SH, Chiou HY, Lin KI, Chang DM, Sytwu HK
Diabetologia 2013 Jan;56(1):136-46

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Duodenal follicular lymphoma lacks AID but expresses BACH2 and has memory B-cell characteristics.

Takata K, Sato Y, Nakamura N, Tokunaka M, Miki Y, Yukie Kikuti Y, Igarashi K, Ito E, Harigae H, Kato S, Hayashi E, Oka T, Hoshii Y, Tari A, Okada H, Al-Kader LA, Maeda Y, Tanimoto M, Kinoshita T, Yoshino T
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2013 Jan;26(1):22-31

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Alternative splicing regulates Prdm1/Blimp-1 DNA binding activities and corepressor interactions.

Morgan MA, Mould AW, Li L, Robertson EJ, Bikoff EK
Molecular and cellular biology 2012 Sep;32(17):3403-13

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Receptor cross-talk spatially restricts p-ERK during TLR4 stimulation of autoreactive B cells.

Lee SR, Rutan JA, Monteith AJ, Jones SZ, Kang SA, Krum KN, Kilmon MA, Roques JR, Wagner NJ, Clarke SH, Vilen BJ
Journal of immunology (Baltimore, Md. : 1950) 2012 Oct 15;189(8):3859-68

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Bim regulates B-cell receptor-mediated apoptosis in the presence of CD40 signaling in CD40-pre-activated splenic B cells differentiating into plasma cells.

Gao Y, Kazama H, Yonehara S
International immunology 2012 May;24(5):283-92

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IL-10+ CTLA-4+ Th2 inhibitory cells form in a Foxp3-independent, IL-2-dependent manner from Th2 effectors during chronic inflammation.

Altin JA, Goodnow CC, Cook MC
Journal of immunology (Baltimore, Md. : 1950) 2012 Jun 1;188(11):5478-88

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Specialized proresolving mediators enhance human B cell differentiation to antibody-secreting cells.

Ramon S, Gao F, Serhan CN, Phipps RP
Journal of immunology (Baltimore, Md. : 1950) 2012 Jul 15;189(2):1036-42

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IL-2 requirement for human plasma cell generation: coupling differentiation and proliferation by enhancing MAPK-ERK signaling.

Le Gallou S, Caron G, Delaloy C, Rossille D, Tarte K, Fest T
Journal of immunology (Baltimore, Md. : 1950) 2012 Jul 1;189(1):161-73

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Soluble factors produced by activated CD4+ T cells modulate EBV latency.

Nagy N, Adori M, Rasul A, Heuts F, Salamon D, Ujvári D, Madapura HS, Leveau B, Klein G, Klein E
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan 31;109(5):1512-7

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Regulatory B10 cells differentiate into antibody-secreting cells after transient IL-10 production in vivo.

Maseda D, Smith SH, DiLillo DJ, Bryant JM, Candando KM, Weaver CT, Tedder TF
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 1;188(3):1036-48

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Identifying regulatory B cells (B10 cells) that produce IL-10 in mice.

Matsushita T, Tedder TF
Methods in molecular biology (Clifton, N.J.) 2011;677:99-111

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NANOS3 function in human germ cell development.

Julaton VT, Reijo Pera RA
Human molecular genetics 2011 Jun 1;20(11):2238-50

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SUMOylation of Blimp-1 promotes its proteasomal degradation.

Shimshon L, Michaeli A, Hadar R, Nutt SL, David Y, Navon A, Waisman A, Tirosh B
FEBS letters 2011 Aug 4;585(15):2405-9

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Blimp1 controls photoreceptor versus bipolar cell fate choice during retinal development.

Brzezinski JA 4th, Lamba DA, Reh TA
Development (Cambridge, England) 2010 Feb;137(4):619-29

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Blimp1-mediated repression of negative regulators is required for osteoclast differentiation.

Nishikawa K, Nakashima T, Hayashi M, Fukunaga T, Kato S, Kodama T, Takahashi S, Calame K, Takayanagi H
Proceedings of the National Academy of Sciences of the United States of America 2010 Feb 16;107(7):3117-22

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RelB NF-kappaB represses estrogen receptor alpha expression via induction of the zinc finger protein Blimp1.

Wang X, Belguise K, O'Neill CF, Sánchez-Morgan N, Romagnoli M, Eddy SF, Mineva ND, Yu Z, Min C, Trinkaus-Randall V, Chalbos D, Sonenshein GE
Molecular and cellular biology 2009 Jul;29(14):3832-44

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Peroxisome proliferator-activated receptor gamma ligands enhance human B cell antibody production and differentiation.

Garcia-Bates TM, Baglole CJ, Bernard MP, Murant TI, Simpson-Haidaris PJ, Phipps RP
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 1;183(11):6903-12

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Recurrent inactivation of the PRDM1 gene in primary central nervous system lymphoma.

Courts C, Montesinos-Rongen M, Brunn A, Bug S, Siemer D, Hans V, Blümcke I, Klapper W, Schaller C, Wiestler OD, Küppers R, Siebert R, Deckert M
Journal of neuropathology and experimental neurology 2008 Jul;67(7):720-7

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Ets-1 regulates plasma cell differentiation by interfering with the activity of the transcription factor Blimp-1.

John SA, Clements JL, Russell LM, Garrett-Sinha LA
The Journal of biological chemistry 2008 Jan 11;283(2):951-62

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Rituximab plus CHOP (R-CHOP) overcomes PRDM1-associated resistance to chemotherapy in patients with diffuse large B-cell lymphoma.

Liu YY, Leboeuf C, Shi JY, Li JM, Wang L, Shen Y, Garcia JF, Shen ZX, Chen Z, Janin A, Chen SJ, Zhao WL
Blood 2007 Jul 1;110(1):339-44

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Cytokine-dependent Blimp-1 expression in activated T cells inhibits IL-2 production.

Gong D, Malek TR
Journal of immunology (Baltimore, Md. : 1950) 2007 Jan 1;178(1):242-52

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Cytokine-dependent Blimp-1 expression in activated T cells inhibits IL-2 production.

Gong D, Malek TR
Journal of immunology (Baltimore, Md. : 1950) 2007 Jan 1;178(1):242-52

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Blimp1 regulates development of the posterior forelimb, caudal pharyngeal arches, heart and sensory vibrissae in mice.

Robertson EJ, Charatsi I, Joyner CJ, Koonce CH, Morgan M, Islam A, Paterson C, Lejsek E, Arnold SJ, Kallies A, Nutt SL, Bikoff EK
Development (Cambridge, England) 2007 Dec;134(24):4335-45

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Toll-like receptor-dependent and -independent viperin gene expression and counter-regulation by PRDI-binding factor-1/BLIMP1.

Severa M, Coccia EM, Fitzgerald KA
The Journal of biological chemistry 2006 Sep 8;281(36):26188-95

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PRDM1/Blimp-1 is expressed in human B-lymphocytes committed to the plasma cell lineage.

Cattoretti G, Angelin-Duclos C, Shaknovich R, Zhou H, Wang D, Alobeid B
The Journal of pathology 2005 May;206(1):76-86

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The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development.

Chang DH, Cattoretti G, Calame KL
Mechanisms of development 2002 Sep;117(1-2):305-9

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The dynamic expression pattern of B lymphocyte induced maturation protein-1 (Blimp-1) during mouse embryonic development.

Chang DH, Cattoretti G, Calame KL
Mechanisms of development 2002 Sep;117(1-2):305-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Detection of Blimp-1 in murine plasmacytoma cell lysate (P3X) using NB 600-235. 1881: murine pre-B cell lysate (negative control). Photo courtesy of DA Savitsky, Columbia University.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells. Expression of EBNA-2 and LMP-1 in IL-21 treated cells. Expression of EBNA-2, LMP-1 and Blimp-1 by immunoblotting; positive control: CBM1-Ral-STO, negative control: Ramos. 1.5x105 cells were loaded in the control lanes and 5x105 were loaded in both untreated and IL-21 treated MEC1 and MEC2 lanes. Note low expression of EBNA-2 and high expression of LMP-1 after IL-21 treatment and induction of Blimp-1 after IL-21 treatment. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0106008), licensed under a CC-BY licence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using paraffin-embedded human tonsil tissue with BLIMP1/PRDM1 antibody at dilution of 1:50. Detection is completed through VC001 (DAB) and Counterstained by hematoxylin; labeling is predominantly in germinal centers. Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - IL-10+Blimp-1-, IL-10+Blimp-1+, IL-10-Blimp-1+ and IL-10-Blimp-1- cells, % total living single splenic CD19+ cells of 3 total mice (Tedder Lab; Duke Univ).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by CD19+ (thick black line) or CD8+ (thin, shaded line). (Tedder Lab; Duke Univ).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Blimp-1 expression by IL-10+ (black line), IL-10- (dotted line) or CD8+ (thin, shaded line) cells (Tedder Lab; Duke Univ)

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - Analysis using the Biotin conjugate of NB600-235. Staining of Blimp-1 in mouse spleen. Image courtesy of product review by Branislav Krljanac.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on U266 cells with BLIMP1/PRDM1 Antibody (3H2-E8) NB600-235 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and permeabilized with 0.1% Saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody (F0101B, R&D Systems).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: BLIMP1/PRDM1 Antibody (3H2-E8) [NB600-235] - An intracellular stain was performed on A431 cells with BLIMP1/PRDM1 [3H2-E8] Antibody NB600-235C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.