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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX100801 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100801, RRID:AB_1951882
- Product name
- 14-3-3 sigma antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Fluoxetine Inhibits DNA Repair and NF-ĸB-modulated Metastatic Potential in Non-small Cell Lung Cancer.
UVB-induced nuclear translocation of TC-PTP by AKT/14-3-3σ axis inhibits keratinocyte survival and proliferation.
Rho kinase inhibitor Y-27632 promotes the differentiation of human bone marrow mesenchymal stem cells into keratinocyte-like cells in xeno-free conditioned medium.
Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway.
The protective effects of spirulina in cyclophosphamide induced nephrotoxicity and urotoxicity in rats.
Wu JY, Lin SS, Hsu FT, Chung JG
Anticancer research 2018 Sep;38(9):5201-5210
Anticancer research 2018 Sep;38(9):5201-5210
UVB-induced nuclear translocation of TC-PTP by AKT/14-3-3σ axis inhibits keratinocyte survival and proliferation.
Kim M, Morales LD, Baek M, Slaga TJ, DiGiovanni J, Kim DJ
Oncotarget 2017 Oct 31;8(53):90674-90692
Oncotarget 2017 Oct 31;8(53):90674-90692
Rho kinase inhibitor Y-27632 promotes the differentiation of human bone marrow mesenchymal stem cells into keratinocyte-like cells in xeno-free conditioned medium.
Li Z, Han S, Wang X, Han F, Zhu X, Zheng Z, Wang H, Zhou Q, Wang Y, Su L, Shi J, Tang C, Hu D
Stem cell research & therapy 2015 Mar 11;6:17
Stem cell research & therapy 2015 Mar 11;6:17
Quercetin 3-O-methyl ether protects FL83B cells from copper induced oxidative stress through the PI3K/Akt and MAPK/Erk pathway.
Tseng HL, Li CJ, Huang LH, Chen CY, Tsai CH, Lin CN, Hsu HY
Toxicology and applied pharmacology 2012 Oct 1;264(1):104-13
Toxicology and applied pharmacology 2012 Oct 1;264(1):104-13
The protective effects of spirulina in cyclophosphamide induced nephrotoxicity and urotoxicity in rats.
Sinanoglu O, Yener AN, Ekici S, Midi A, Aksungar FB
Urology 2012 Dec;80(6):1392.e1-6
Urology 2012 Dec;80(6):1392.e1-6
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 14-3-3 sigma antibody detects SFN protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g HeLa whole cell lysate/extract D. 30 ?g HepG2 whole cell lysate/extractE. 30 ?g A375 whole cell lysate/extract12% SDS-PAGE14-3-3 sigma antibody (GTX100801) dilution: 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 14-3-3 sigma antibody detects SFN protein by western blot analysis.A. 30 ?g Neuro2A whole cell lysate/extractB. 30 ?g GL261 whole cell lysate/extract C. 30 ?g C8D30 whole cell lysate/extract12% SDS-PAGE14-3-3 sigma antibody (GTX100801) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 sigma antibody (GTX100801) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 14-3-3 sigma antibody detects 14-3-3 sigma protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: 14-3-3 sigma protein stained by 14-3-3 sigma antibody (GTX100801) diluted at 1:500.Blue: Hoechst 33343 staining.
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- 14-3-3 sigma antibody immunoprecipitates SFN protein in IP experiments.IP samples: A431 whole cell extractA. 40 £gg A431 whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of SFN protein by 4 £gg 14-3-3 sigma antibody (GTX100801)5 % SDS-PAGEThe immunoprecipitated SFN protein was detected by 14-3-3 sigma antibody (GTX100801) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using 14-3-3 sigma(GTX100801) antibody at 1:100 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- 14-3-3 sigma antibody detects 14-3-3 sigma protein at cytosol on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. 14-3-3 sigma antibody (GTX100801) dilution: 1:500.
