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- Antigen structure
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- Product number
- STJ70838 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-14-3-3 sigma/Stratifin antibody (Internal) (STJ70838)
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody anti-14-3-3 sigma/Stratifin (Internal) is suitable for use in ELISA and Western Blot research applications.
- Reactivity
- Human, Mouse, Rat, Canine, Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
DLHTLSEDSYKDST- Epitope
- NA
- Isotype
- IgG
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20 on receipt and minimise freeze-thaw cycles.
- Handling
- NA
No comments: Submit comment
Supportive validation
Supportive validation
Supportive validation
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70838 (0. 03µg/ml) staining of Human Skin lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70838 (0. 03µg/ml) staining of A431 (A) and U2OS (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70838 (0. 03µg/ml) staining of Mouse (A) and Rat (B) Skin lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- STJ70838 (2µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
