LS-C818411

antibody from LSBio

Targeting: SFN YWHAS

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

LS-C818411

Provider

LSBio

Product name

SFN / Stratifin / 14-3-3 Sigma Antibody (Preservative Free) LS-C818411

Antibody type

Polyclonal

Description

Protein A affinity chromatography

Reactivity

Mouse

Host

Rabbit

Isotype

IgG

Storage

Store at 2°C to 8°C for up to 1 month. Aliquot and store at -20°C to -80°C for up to 1 year. Avoid freeze/thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Mouse SFN was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. 4 uL anti-Mouse SFN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Mouse SFN rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Mouse SFN was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. 4 uL anti-Mouse SFN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Mouse SFN rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Mouse SFN was immunoprecipitated using: Lane A: 0.5 mg NIH3T3 Whole Cell Lysate. 4 uL anti-Mouse SFN rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-Mouse SFN rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 30 kDa. Observed band size: 30 kDa.