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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
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- Product number
- MA5-34912 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-31 Monoclonal Antibody (8-C3)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Recombinant protein lysate, A431, LOVO, MCF-7, human tonsil tissue, human spleen tissue.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 8-C3
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL-31 in recombinant protein lysate. Samples were incubated with IL-31 monoclonal antibody (Product # MA5-34912), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IL-31 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with IL-31 monoclonal antibody (Product # MA5-34912), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IL-31 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with IL-31 monoclonal antibody (Product # MA5-34912), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IL-31 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with IL-31 monoclonal antibody (Product # MA5-34912), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IL-31 in paraffin-embedded human tonsil tissue. Samples were incubated with IL-31 monoclonal antibody (Product # MA5-34912), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of IL-31 in paraffin-embedded human spleen tissue. Samples were incubated with IL-31 monoclonal antibody (Product # MA5-34912), and followed by hematoxylin.
