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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-534 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-534, RRID:AB_631445
- Product name
- Anti-EPS15
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Identification of c-Src tyrosine kinase substrates in platelet-derived growth factor receptor signaling.
A regulated interaction with the UIM protein Eps15 implicates parkin in EGF receptor trafficking and PI(3)K–Akt signalling
A method to estimate the somatic cell count of milk from a mastitic quarter using composite somatic cell count.
Amanchy R, Zhong J, Hong R, Kim JH, Gucek M, Cole RN, Molina H, Pandey A
Molecular oncology 2009 Dec;3(5-6):439-50
Molecular oncology 2009 Dec;3(5-6):439-50
A regulated interaction with the UIM protein Eps15 implicates parkin in EGF receptor trafficking and PI(3)K–Akt signalling
Lara Fallon, Catherine M.L. Bélanger, Amadou T. Corera, Maria Kontogiannea, Elsa Regan-Klapisz, France Moreau, Jarno Voortman, Michael Haber, Geneviève Rouleau, Thorhildur Thorarinsdottir, Alexis Brice, Paul M.P. van Bergen en Henegouwen, Edward A. Fon
Nature Cell Biology 2006 Jul;8(8):834-842
Nature Cell Biology 2006 Jul;8(8):834-842
A method to estimate the somatic cell count of milk from a mastitic quarter using composite somatic cell count.
Thurmond MC
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire 1990 Jan;54(1):190-4
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire 1990 Jan;54(1):190-4
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 219, 111, 83, 48, 32, 26, 17
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 99
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
