Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-30138 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PSMD12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.66 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PSMD12 using 30 µg of A) NT2D1 (B) IMR32 and C) U87-MG lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a PSMD12 polyclonal antibody (Product # PA5-30138) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PSMD12 Polyclonal Antibody (Product # PA5-30138). Sample (30 µg of whole cell lysate). Lane A: NT2D1. Lane B: IMR32. Lane C: U87-MG. 10% SDS PAGE. PSMD12 Polyclonal Antibody (Product # PA5-30138) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human breast cancer, using PSMD12 (Product # PA5-30138) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.