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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28051 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-1RAcP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2 membrane extract, mouse liver.
- Concentration
- 0.36 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL1RAP using 30 µg of Jurkat lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an IL1RAP polyclonal antibody (Product # PA5-28051) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IL-1RAcP was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a IL-1RAcP Polyclonal Antibody (Product # PA5-28051) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IL-1RAcP was performed by separating 30 µg of HepG2 whole cell and membrane extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a IL-1RAcP Polyclonal Antibody (Product # PA5-28051) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IL-1RAcP Polyclonal Antibody detects IL1RAP protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. IL1RAP stained by IL-1RAcP Polyclonal Antibody (Product # PA5-28051) diluted at 1:300. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
