NB200-210

antibody from Novus Biologicals

Targeting: EP400 CAGH32, DKFZP434I225, KIAA1498, KIAA1818, P400, TNRC12

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NB200-210

Provider

Novus Biologicals

Proper citation

Novus Cat#NB200-210, RRID:AB_10001338

Product name

Rabbit Polyclonal p400 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Basal activity of a PARP1-NuA4 complex varies dramatically across cancer cell lines.

Krukenberg KA, Jiang R, Steen JA, Mitchison TJ
Cell reports 2014 Sep 25;8(6):1808-1818

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PRC1 and PRC2 are not required for targeting of H2A.Z to developmental genes in embryonic stem cells.

Illingworth RS, Botting CH, Grimes GR, Bickmore WA, Eskeland R
PloS one 2012;7(4):e34848

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The p400 ATPase regulates nucleosome stability and chromatin ubiquitination during DNA repair.

Xu Y, Sun Y, Jiang X, Ayrapetov MK, Moskwa P, Yang S, Weinstock DM, Price BD
The Journal of cell biology 2010 Oct 4;191(1):31-43

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NCoR1 mediates papillomavirus E8;E2C transcriptional repression.

Powell ML, Smith JA, Sowa ME, Harper JW, Iftner T, Stubenrauch F, Howley PM
Journal of virology 2010 May;84(9):4451-60

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Genome-wide siRNA screen identifies SMCX, EP400, and Brd4 as E2-dependent regulators of human papillomavirus oncogene expression.

Smith JA, White EA, Sowa ME, Powell ML, Ottinger M, Harper JW, Howley PM
Proceedings of the National Academy of Sciences of the United States of America 2010 Feb 23;107(8):3752-7

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VHL loss actuates a HIF-independent senescence programme mediated by Rb and p400.

Young AP, Schlisio S, Minamishima YA, Zhang Q, Li L, Grisanzio C, Signoretti S, Kaelin WG Jr
Nature cell biology 2008 Mar;10(3):361-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: p400 Antibody [NB200-210] - Whole cell lysate from CT26.WT (15 and 50 ug) cells. Antibodies: Affinity purified rabbit anti-p400 antibody used for WB at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: p400 Antibody [NB200-210] - Detection of Human p400 on HeLa whole cell lysate using NB200-210. Immunoprecipitation of p400 was also accomplished, albeit less efficiently, using another rabbit anti-p400 antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: p400 Antibody [NB200-210] - Detection of human p400 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, K-562, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p400 antibody NB200-210 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: p400 Antibody [NB200-210] - Detection of mouse p400 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-p400 antibody NB200-210 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: p400 Antibody [NB200-210] - Detection of human p400 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-p400 antibody NB200-210 (lot 2) used for IP at 6 ug per reaction. p400 was also immunoprecipitated by a previous lot of this antibody (lot 1) and rabbit anti-p400 antibody from Company B. For blotting immunoprecipitated p400, NB200-210 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.